Sodium permanganate

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to terrestrial arthropods: long-term

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

See the read-across report attached in Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 100 mg/kg bw

Nominal / measured:

meas. (not specified)

Conc. based on:

other: Mn in soil

Basis for effect:

mortality

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 067 mg/kg bw

Nominal / measured:

not specified

Conc. based on:

other: Mn

Basis for effect:

reproduction

Reference 2

Endpoint:

toxicity to terrestrial arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

See the read-across report attached in Section 13.

Qualifier:

according to guideline

Guideline:

ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)

Deviations:

yes

Remarks:

The method was adapted for use with natural soils

GLP compliance:

not specified

Application method:

soil

Analytical monitoring:

yes

Details on preparation and application of test substrate:

The soil was a natural soil containing 1.2% organic matter.

Test organisms (species):

Folsomia candida

Animal group:

Acari (soil-dwelling predatory mite)

Study type:

laboratory study

Limit test:

no

Total exposure duration:

28 d

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 100 mg/kg bw

Nominal / measured:

meas. (not specified)

Conc. based on:

other: Mn in soil

Basis for effect:

mortality

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

1 067 mg/kg bw

Conc. based on:

other: Mn

Basis for effect:

reproduction

The natural background Mn concentration determined in the negative control treatment was 94 mg kg-I. Total extractable Mn concentrations (in excess of background) in the experimental treatments ranged from 99 to 140% and averaged 111% of nominal. Exchangeable Mn fraction expressed as percent of total concentration increased with increasing soil Mn loads.

Range finding test:

Manganese sulphate monohydrate (MnSO4·H2O) was used to conduct a range finding test. Adult survival and juvenile production decreased significantly at 5,000 mg/kg. There were no juveniles above the 5,000 mg/kg treatment concentration.

Definitive test:

Manganese did not affect (P = 0.168) adult springtail survival at the 1667 mg/kg concentration. Adult survival was significantly reduced (P = 0.0001) at 2444 mg/kg. The bounded NOEC for juvenile production was 1067 mg/kg (P=0.070). The bounded LOEC for juvenile production was 1100 mg/kg (P=0.025). No juveniles were produced in 2444 mg/kg treatment. The

EC20 and EC50 values for Mn for juvenile production were 1209 and 1663 mg/kg, respectively.

Table 1: Nominal and measured concentrations of manganese

Nominal (mg/kg)	Measured (mg/kg)	Corrected recovery (%)
0	94	0
287	386	102
500	633	108
695	1067	140
966	1100	104
1343	1667	117
1867	2444	126
2594	2836	106
3606	3667	99
5056	5056	99

Validity criteria fulfilled:

yes

Remarks:

apart from adult mortality greater than 30% at concentrations higher than 1667 mg/kg and higher concentrations and mean number of juveniles was less than 80 at concentrations higher than 1100 mg/kg. Not considered to affect choice of NOEC.

Conclusions:

Under the conditions of the study, the No Observable Effect Concentration (NOEC) is 1067 mg Mn/kg dw.

Description of key information

Under the conditions of the study, the No Observable Effect Concentration (NOEC) for the read across source substance is 1 067 mg Mn/kg dw.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil dwelling arthropods:

1 067 mg/kg soil dw

Additional information

Under the conditions of the study, the No Observable Effect Concentration (NOEC) for the read across source substance is 1 067 mg Mn/kg dw.  The study was awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997).