1-diethylaminopropan-2-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-diethylaminopropan-2-ol

EC Number:

224-537-7

EC Name:

1-diethylaminopropan-2-ol

Cas Number:

4402-32-8

Molecular formula:

C7H17NO

IUPAC Name:

1-(diethylamino)propan-2-ol

Test material form:

liquid

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 1°C

pH:

7.8 - 8.1

Nominal and measured concentrations:

100, 55.6, 30.9, 17.1, 9.53 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed without headspace
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16h light / 8h dark
- Light intensity: room light

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: pH adjusted to 7.8 - 8.1

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

31.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: pH unadjusted 9.7

Details on results:

Because the pH in the highest test concentration exceeded 9.0 in the preliminary study, 100 mg/L adjusted to the pH of dilution water was added to confirm whether the effect in a test where pH was not adjusted was caused by the test item or by the high pH. As a result, the 48-hour EC50 in pH unadjusted exposure test was determined to be 31.3 mg/L (95% confidence limits: 26.4-37.6 mg/L). In contrast, no adverse effect was found in the test where pH was adjusted.
From the results above, it was concluded that pH had an adverse acute effect on the test organisms.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24-hour and 48-hour EC50 of the test item for Daphnia magna in pH adjusted exposure test were both > 100 mg/L.