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EC number: 606-059-4 | CAS number: 185756-31-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 Jul 2004 to 14 Jul 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April, 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- December, 1992
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- Public Draft, April 1996
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Version / remarks:
- 1989
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/DIS 10706 Water quality - Determination of long term toxicity of substances to Daphnia magna STRAUS
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Appearance: White solid
- Water-solubility: 29 mg/L (20°C)
- Homogeneity: homogeneous
- Storage conditions: ambient - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L
- Sampling method: At the begin (after 0 h) samples from vessels without daphnids and at the end of the exposition (after 48 h) samples from vessels with daphnids were analysed.
- Sample storage conditions before analysis: ambient (samples were analysed at the sampling date) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Preparation of the stock solution: The test substance was stirred in M4 medium tor about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by filtration with a membrane filter (pore width 0.2 µm). The filtrate was a clear and colourless test solution. The concentration of the stock solution was 200.1 mg/2L.
- Preparation test solutions: By diluting this filtrate of the stock solution with M4 medium the test solutions were prepared.
- Controls: M4 medium - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously inhouse.
- Age of the animals at the begin of exposure: 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals: 2 - 4 weeks
- Feeding during test: None
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.43 mmol/L (total hardness)
- Test temperature:
- 20.0 - 20.4 °C
- pH:
- 8.0 - 8.2
- Dissolved oxygen:
- 8.4 - 9.0 mg/L
- Conductivity:
- 606 µS/cm
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 12.5, 25, 50 and 100 mg/L (based on a range-finding test)
- Measured concentrations (t = 0h): n.d., 2.94, 5.88, 11.63, 21.9 mg/L
- Measured concentrations (t = 48h): n.d., 2.81, 5.68, 11.21, 22.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- Test volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (M4 medium) was used for culture and test purposes.
- Total hardness: 2.20 - 3.20 mmol/L
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
- Molar ratio Ca:Mg: about 4:1
- pH-value: 7.5-8.5
- Conductivity: 550 - 650 µS/cm
- Culture medium different from test medium: No
- Aeration of medium: after preparation the M4 medium is aerated for approximately 24 h until saturation with oxygen is reached.
OTHER TEST CONDITIONS
- Illumination: Artificial light, type warm white (OSRAM L58 W31), day:night rhythm = 16 : 8 h
- Light intensity: About 1 - 8 µEI(m².s) at a wave length of 400 - 750 nm
EFFECT PARAMETERS MEASURED:
- Test parameter: Swimming ability of the test animals; recorded visually after 0, 24 and 48 h
- Other: pH and oxygen were determined at test start and at the end of exposure
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 22.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: no effects up to the limit of water solubility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: no effects up to the limit of water solubility
- Details on results:
- Up to the highest test concentration of 22.8 mg/L (nominal 100 mg/L) no effect on mobility of the daphnids was observed.
- Results with reference substance (positive control):
- The 24-h EC50 value of the reference substance was 1.41 mg/L.
- Reported statistics and error estimates:
- No effects on daphnid mobility were observed; no statistics were required to derive the effect concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is with high probability not acutely harmful to aquatic invertebrates.
Reference
Description of key information
The test substance is with high probability not acutely harmful to aquatic invertebrates.
Key value for chemical safety assessment
Additional information
The acute toxicity to aquatic invertebrates was determined in a study according to OECD TG 202 and in compliance with GLP criteria. In this study daphnids (D. magna, 20 per concentration) were exposed to dilutions of an aqueous eluate (filtrate) of the test substance stock solution of 100 mg/L, at nominal concentrations of 0 (control), 12.5, 25, 50 and 100 mg/L for 48 hours under static conditions. The mean analytical recovery rate for all test concentrations was 22.8%. Effect concentrations were corrected for the recovery rate. Immobilization was recorded after 24 and 48 hours exposure. No immobilization was observed in the control and test groups. Based on these findings the 48-h EC50 value was determined at > 22.8 mg/L (100 mg/L nominal). The test substance showed no effects up to the limit of water solubility.
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