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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Mar 01, 2011 - April 05, 2011
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Batch: EF10005270
ThOD: 2.693 mg O2/mg
Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant
- OriginMunicipal sewage treatment plant
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice.
- Concentration of suspended solids per litre: 30 mg
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
According to guideline
Reference substance:
Test performance:
The reference item (Aniline) was sufficiently degraded to 71 % after 14 days and to 77% after 28 days of incubation.The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
Key result
% degradation (O2 consumption)
Sampling time:
28 d
Key result
% degradation (test mat. analysis)
Sampling time:
28 d
Results with reference substance:
14d: 96 %, 28d: 74 %
Validity criteria fulfilled:
Interpretation of results:
not readily biodegradable
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 301C. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.

Description of key information

GLP OECD 301C: not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information