4-amino-N-(4-aminophenyl)benzenesulphonamide

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Data is from study report.

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: Test chemical concentration used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively.
- Sampling method: The suspension were made from the stock solutions (1000 mg/l).

Vehicle:

not specified

Test organisms (species):

other: Scenedesmus subspicatus

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: SAG 88.81
- Other: The algae culture is set at 10,000 cells/ml.

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

20⁰C (293 K)

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Nominal and net volume of the test vessel were 250 and 100 ml, respectively.
- Equipment settings: GAIN X 1
- Self-staining of the sample: reddish brown

GROWTH MEDIUM
- Standard medium used: Yes, OECD medium was used as a test medium during the study.

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

152.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

37.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

21.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

21.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Experimental results   Measuring time: 96 h

Conc. in mg/l	Fluorometer values			% of the control
	Inoculated	Uninoculated	Difference
1.953 3.906 7.813 15.625 31.25 62.5 125 250 Control	790 735 731 626 229 101 70 33 957	1 0 1 1 3 5 8 15 1	789 735 730 625 226 96 62 18	82.51 75.82 76.34 65.36 23.61 10.04 6.48 1.88

EC20 = 2.68 mg/l

EC50 = 21.4 mg/l

EC90 = 83.2 mg/l

Conc. in ml/l	Fluorometer units			Standard deviation	Variance in %
	Single value	Mean	Net value
1.953 Inoculated     Uninoculated   3.906 Inoculated     Uninoculated   7.813 Inoculated     Uninoculated   15.625 Inoculated     Uninoculated   31.25 Inoculated     Uninoculated   62.5 Inoculated     Uninoculated   125 Inoculated     Uninoculated   250 Inoculated     Uninoculated   Blank values Inoculated     Uninoculated	821.9 758 817 753.4 0.8 1.4 733.8 741.6 710.4 716.4 0.8 1 760.5 688.3 740.4 734.9 2.7 1.2 665.5 652.9 625.9 561.5 1.1 1.3 320.7 201.4 185.6 210.4 3.9 3.7 112. 94 98.8 100.4 6.7 5.1 72.8 67.7 64.9 75.3 8.6 8.2 30.2 37.7 32.1 32.4 14.7 15.5 874.5 1037.3 985 933.2 1.7 0.9	790       1   725       0   731       1   626       1   229       3   101       5   70       8   33       15   957     1	789           725           730           625           226           96           62           18	34.5           14.59           30.53           46.34           61.6           7.68           4.73           3.22           69.77	4.37           2.01           4.18           7.4           26.86           7.58           6.74           9.72           7.29

The standard deviation is determined from the individual values.

Experimental results   Measuring time: 72 h

Conc. in mg/l	Fluorometer values			% of the control
	Inoculated	Uninoculated	Difference
1.953 3.906 7.813 15.625 31.25 62.5 125 250 Control	507 437 417 437 166 88 67 34 658	1 0 1 1 2 5 7 13 1	506 437 416 436 184 83 80 21	77.03 86.52 83.33 88.37 24.97 12.64 9.13 3.12

EC20 = ----

EC50 = 21.8 mg/l

EC90 = 109.5 mg/l

Conc. in ml/l	Fluorometer units			Standard deviation	Variance in %
	Single value	Mean	Net value
1.953 Inoculated     Uninoculated   3.906 Inoculated     Uninoculated   7.813 Inoculated     Uninoculated   15.625 Inoculated     Uninoculated   31.25 Inoculated     Uninoculated   62.5 Inoculated     Uninoculated   125 Inoculated     Uninoculated   250 Inoculated     Uninoculated   Blank values Inoculated       Uninoculated	532.7 485.8 525.4 485.7 0.8 1.8 453.7 411.1 449.4 437.5 0.8 1 437.4 425.5 426.7 381.1 1.4 0.9 359.9 392.1 412.8 594.2 13 1.4 171.3 132.2 150.7 211.3 1.8 3.8 95.3 80.2 86.1 93.1 5.5 5.6 72.2 63.8 65.3 67.7 8 7.8 34.9 33.3 38 33.7 13.5 13.4 595.8 623.1 720.9 693.5 0.9 1.5	507       1   437       0   417       1   437       1   166       2   88       5   67       7   34       13   658     1	506           437           416           436           164           83           60           21	25.18           18.15           24.96           106.37           33.84           6.88           3.67           2.13           58.37	4.96           4.37           5.98           24.44           20.4           7.76           5.46           6.08           3.87

Experimental results   Measuring time: 48 h

Conc. in mg/l	Fluorometer values			% of the control
	Inoculated	Uninoculated	Difference
1.953 3.906 7.813 15.625 31.25 62.5 125 250 Control	251 213 190 186 146 83 65 39 264	1 0 1 1 2 5 7 10 1	250 213 189 185 144 78 58 29	84.81 80.87 71.75 74.03 54.67 29.81 22.02 11.01

 

EC20 = 4.28 mg/l

EC50 = 37.1 mg/l

EC90 = ----

Conc. in ml/l	Fluorometer units			Standard deviation	Variance in %
	Single value	Mean	Net value
1.953 Inoculated     Uninoculated   3.906 Inoculated     Uninoculated   7.813 Inoculated     Uninoculated   15.625 Inoculated     Uninoculated   31.25 Inoculated     Uninoculated   62.5 Inoculated     Uninoculated   125 Inoculated     Uninoculated   250 Inoculated     Uninoculated   Blank values Inoculated     Uninoculated	263.6 244.7 270.5 228.5 1.3 1.4 229.2 210.8 206.6 208.1 1.4 0.4 187.2 203.1 195.7 176.4 1.3 1.2 234.1 204.8 178.7 168.4 1.1 1.4 145.2 165.4 136.1 138.5 2.2 1.9 78.3 77.1 81.5 95.9 5.1 5 65.3 61.6 66.4 68.2 7.9 7.1 37.4 43.7 41.6 36.7 9.7 10.8 232.6 260.1 268.9 298 1.9 1.1	251       1   213       0   190       1   198       1   145       2   83       5   65       7   39       10   204     1	250           213           188           195           144           78           58           29	18.99           10.48           11.48           29.39           13.3           8.67           2.79           3.36           26.84	7.54           4.91           8.02           14.35           9.09           10.42           4.26           8.42           10.17

Experimental results   Measuring time: 24 h

Conc. in mg/l	Fluorometer values			% of the control
	Inoculated	Uninoculated	Difference
1.953 3.906 7.813 15.625 31.25 62.5 125 250 Control	78 64 67 89 61 53 47 36 81	2 1 1 1 1 1 4 7 1	76 63 66 68 60 52 43 28	85.12 78.85 82.8 85.11 75.09 65.08 53.82 36.3

 

EC20 = 3.77 mg/l

  5.1 mg/l

             23.6 mg/l

EC50 = 152.2 mg/l

EC90 = ----

Conc. in ml/l	Fluorometer units			Standard deviation	Variance in %
	Single value	Mean	Net value
1.953 Inoculated     Uninoculated   3.906 Inoculated     Uninoculated   7.813 Inoculated     Uninoculated   15.625 Inoculated     Uninoculated   31.25 Inoculated     Uninoculated   62.5 Inoculated     Uninoculated   125 Inoculated     Uninoculated   250 Inoculated     Uninoculated   Blank values Inoculated     Uninoculated	88.7 78.6 82.8 85.3 2 2.9 68.9 61.5 63.1 66.1 1.1 1.2 64.4 77.5 82.2 86.3 2.2 1.4 83.9 81.8 66.9 67 1.7 1.1 58.8 57.2 62.1 66.1 1.1 1 48.9 49.8 55.3 61.3 2.7 1.2 45.6 45.1 45.6 52.2 5.1 3.2 35.8 33.8 41.5 34.3 7.4 7 73.3 78.8 93.8 80.7 1.7 1.9	78       2   64       1   67       1   68       1   61       1   53       1   47       4   36       7   81     1	76           63           66           68           60           52           43           29	10           3.28           6.81           9.64           3.94           6.02           3.39           3.43           8.69	12.77           5.05           10.07           13.8           6.45           11.18           7.2           9.4           10.64

Experimental results   Measuring time: 0 h

Conc. in mg/l	Fluorometer values			% of the control
	Inoculated	Uninoculated	Difference
1.953 3.906 7.813 15.625 31.25 62.5 125 250 Control	25 23 22 23 25 25 25 29 25	2 2 1 2 2 1 4 6 3	23 21 21 21 23 24 21 23	102.79 93.85 93.85 93.85 102.79 107.26 93.85 102.79

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect of test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.

Executive summary:

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs.The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

21.4 mg/L

Additional information

Short term toxicity to Scenedesmus subspicatus was carried out for 96 hrs (Sustainability Support Services (Europe) AB, DUU/WL-A 520, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Scenedesmus subspicatus in a fresh water system at a temperature of 20⁰C (293 K). Scenedesmus subspicatus SAG 88.81 was used as a test organism. The algae culture is set at 10,000 cells/ml.The suspension were made from the stock solutions (1000 mg/l). Test chemical nominal concentrations used for the study were 0, 1.953, 3.906, 7.813, 15.625, 31.25, 62.5, 125 and 250 mg/l, respectively. Standard OECD medium was used as a test medium during the study. Based on the effect of test chemical 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide on test organism Scenedesmus subspicatus, the 24, 48, 72 and 96 hrs EC50 value was determined to be 152.2, 37.1, 21.8 and 21.4 mg/l, respectively.Thus, based on the 96 hr EC50 value (21.4 mg/l), it can be concluded that the substance 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.