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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

in vitro cytogenicity / chromosome aberration study in mammalian cells
Sister-Chromatid Exchanges in human lymphocytes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies

Data source

Reference Type:
In vitro studies of biological effects of cigarette smoke condensate. II. Induction of sister-chromatid exchanges in human lymphocytes by weakly acidic, semivolatile constituents.
Tommy Jansson et al.

Materials and methods

GLP compliance:
Type of assay:
sister chromatid exchange assay in mammalian cells

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Test material form:
Details on test material:
QSAR method used


Species / strain
Species / strain / cell type:
lymphocytes: human
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
Positive control substance:
other: styrene-7,8-oxide
Details on test system and experimental conditions:
Blood from healthy non-smoking donors was
centrifuged (250 × g) to remove the erythrocytes
and the supernatant was collected. The lymphocyte
fraction thus obtained was grown in Medium
199 with Earles salt. The cultures contained 25%
autologous serum, 1.25% phytohaemagglutinin
(Reagent grade, Wellcome Reagents Ltd., London,
England) and 0.1 mM 5-bromodeoxyuridine. After
24 h incubation at 37°C, the fractions or compounds
to be tested were added to the cultures. In
the tests of pure compounds, the potent SCE
inducer styrene-7,8-oxide (Norppa et al., 1980)
was used as a positive control. The final concentrations
of the solvents, DMSO, EtOH or
DMSO/EtOH (1:1) did not exceed 0.66%, 1.0%
and 0.5%, respectively. After 88-90 h, the cells
were treated consecutively with colchicine (50
ng/ml, 2 h) and hypotonic KC1 (0.075 M, 5-10
min) and they were then fixed in methanol/acetic
acid (3 : 1) for 1 h. After the culture time used, the
portion of cells that have divided more than 2
times is about 20-30%. Chromosome preparations
were made by applying the cell suspension to wet
cooled slides. The staining procedure was mainly
according to Wolff and Perry (1974). The slides
were stained with Hoechst dye (0.5 #g/ml) for 12
min and then rinsed in Mcllvaines buffer (pH 7.0).
They were then exposed to UV-light (Philips TUV
30W G30T8, 10 cm) for 10 min, incubated in 0.3
M NaCI/0.3 M sodium citrate for 2 h at 60°C and
stained with Giemsa dye (4%, pH 6.8) for 20 min.
Well-spread metaphases were scored on coded
slides and 25 metaphases from one culture were
analysed for each concentration tested.

Results and discussion

Test results
Key result
Species / strain:
Metabolic activation:
not specified
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified

Applicant's summary and conclusion

Under the tested conditions, the Sister-chromatid exchange (SCE) test in human lymphocytes revealed no positive result of 2-(2-propenyl)phenol (CAS 1745-81-9 ) to induce SCE.