2-allylphenol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Sister-Chromatid Exchanges in human lymphocytes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1986

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

publication

Title:

In vitro studies of biological effects of cigarette smoke condensate. II. Induction of sister-chromatid exchanges in human lymphocytes by weakly acidic, semivolatile constituents.

Author:

Tommy Jansson et al.

Year:

1986

Materials and methods

GLP compliance:

no

Type of assay:

sister chromatid exchange assay in mammalian cells

Test material

Test material information

Constituent 1

Reference substance name:

2-allylphenol

EC Number:

217-119-0

EC Name:

2-allylphenol

Cas Number:

1745-81-9

Molecular formula:

C9H10O

IUPAC Name:

2-(prop-2-en-1-yl)phenol

Test material form:

liquid

Details on test material:

QSAR method used

Method

Species / strain

Species / strain / cell type:

lymphocytes: human

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: styrene-7,8-oxide

Details on test system and experimental conditions:

Blood from healthy non-smoking donors was
centrifuged (250 × g) to remove the erythrocytes
and the supernatant was collected. The lymphocyte
fraction thus obtained was grown in Medium
199 with Earles salt. The cultures contained 25%
autologous serum, 1.25% phytohaemagglutinin
(Reagent grade, Wellcome Reagents Ltd., London,
England) and 0.1 mM 5-bromodeoxyuridine. After
24 h incubation at 37°C, the fractions or compounds
to be tested were added to the cultures. In
the tests of pure compounds, the potent SCE
inducer styrene-7,8-oxide (Norppa et al., 1980)
133
was used as a positive control. The final concentrations
of the solvents, DMSO, EtOH or
DMSO/EtOH (1:1) did not exceed 0.66%, 1.0%
and 0.5%, respectively. After 88-90 h, the cells
were treated consecutively with colchicine (50
ng/ml, 2 h) and hypotonic KC1 (0.075 M, 5-10
min) and they were then fixed in methanol/acetic
acid (3 : 1) for 1 h. After the culture time used, the
portion of cells that have divided more than 2
times is about 20-30%. Chromosome preparations
were made by applying the cell suspension to wet
cooled slides. The staining procedure was mainly
according to Wolff and Perry (1974). The slides
were stained with Hoechst dye (0.5 #g/ml) for 12
min and then rinsed in Mcllvaines buffer (pH 7.0).
They were then exposed to UV-light (Philips TUV
30W G30T8, 10 cm) for 10 min, incubated in 0.3
M NaCI/0.3 M sodium citrate for 2 h at 60°C and
stained with Giemsa dye (4%, pH 6.8) for 20 min.
Well-spread metaphases were scored on coded
slides and 25 metaphases from one culture were
analysed for each concentration tested.

Results and discussion

Test results

Key result

Species / strain:

lymphocytes:

Metabolic activation:

not specified

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Applicant's summary and conclusion

Conclusions:

Under the tested conditions, the Sister-chromatid exchange (SCE) test in human lymphocytes revealed no positive result of 2-(2-propenyl)phenol (CAS 1745-81-9 ) to induce SCE.