2-allylphenol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Acute Toxicity of Polar Narcotics to Three Aquatic Species (Daphnia magna, Poecilia reticulata and Lymnaea stagnalis) and Its Relation to Hydrophobicity .

Author:

Ramos,E.U., C. Vermeer, W.H.J. Vaes, and J.L.M. Hermens

Year:

1998

Bibliographic source:

Chemosphere37(4): 633-650

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-allylphenol

EC Number:

217-119-0

EC Name:

2-allylphenol

Cas Number:

1745-81-9

Molecular formula:

C9H10O

IUPAC Name:

2-(prop-2-en-1-yl)phenol

Details on test material:

- Name of test material (as cited in study report): 2-allylphenol
- Analytical purity: 98%
- Other: Supplier: Aldrich-Chemie, Steinheim, Germany

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Chemical analyses of water samples were conducted before and after each renewal.

Test solutions

Vehicle:

yes

Details on test solutions:

Toxicity to P. reticulata was tested in 96 h lethality tests, carried out using a semi-static system (24 h renewal). 10 fish were exposed to 1 L test solution in 1.5 L jars. Stock solutions were made in iso-propanol for each concentration. Test solutions were prepared 24 h in advance by spiking 0.5 mL iso-propanol stock to 1 L CFW, and stirring until use. Control animals were exposed to 0.5 mL/L iso-propanol.
Test concentration ranges were attempted to be from 4xEC50 to EC50/4, where EC50 values were collected from literature, or estimated from pilot experiments or QSAR.

Test organisms

Test organisms (species):

Poecilia reticulata

Details on test organisms:

Guppies were cultured at 23 ± 1 °C under a 12 h photoperiod. Animals were fed everyday with commercial fish food. 2-3 months-old (1.8 ± 0.3 cm and 69 ± 34 mg) female animals were used in the lethality tests.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20-25°C

pH:

7.1-8.2

Dissolved oxygen:

0.9-9.5

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The average of all measured concentrations was used.