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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1981-08-13
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: no GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ETAD Fermentation Tube Method
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-xylidine
EC Number:
202-440-0
EC Name:
2,4-xylidine
Cas Number:
95-68-1
Molecular formula:
C8H11N
IUPAC Name:
2,4-dimethylaniline

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified
Details on test solutions:
- Stock solution: 10 g/L in de-ionized water, stirred over a period of 24 hours at room-temperature (approx. 20 - 22 °C) and a pH of 2.3.
- Analyses of the stock solution: 7568 mg dissolved organic carbon / L (DOC)
(calculated value: 7929 mg C / g)
26200 mg chemical oxygen demand / L (COD)

Test organisms

Test organisms (species):
sewage, domestic
Details on inoculum:
- As inoculum sewage sludge from a domestic sewage treatment plant was used. During pre-incubation the suspension was only very slightly stirred, but an oxygen free atmosphere was not used.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24
Remarks on exposure duration:
37° C

Test conditions

Hardness:
no data
Test temperature:
21 +/- 1 °C
pH:
not recorded
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
no data
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
150 mg/L
Conc. based on:
test mat.
Basis for effect:
other: gas production

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
A validation of the results is not possible, as neither validation crieria had been documented, nor the absolute values of the gas production activities had been recorded within all the laboratory manuscripts of the fermentation tube tests. No classification can be made on the base of this result.
Executive summary:

Product has not to be classified.