Hexachlorocyclopentadiene

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Deviations:

yes

Remarks:

No depuration phase, no sample of fish until the end of the uptake phase

GLP compliance:

no

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: End of the study
- Sampling intervals/frequency for test medium samples: Daily

Vehicle:

not specified

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION OF SPIKED FISH FOOD
- Details on fish food (source, fat content as supplied, etc): brine shrimp nauplii 3 to 4 times a day

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Strain: Pimephales promelas
- Age at study initiation: 1 day

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

30 d

Hardness:

45 to 47 mg/l (CaCO3)

Test temperature:

25°C +/- 2°C

pH:

7.2 to 7.7

Dissolved oxygen:

7.2 to 8.6 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, 45x16x18 cm, 8.9 l
- No. of organisms per vessel: 25
- Renewal rate of test solution (frequency/flow rate): 500 ml / 3 min

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Unfiltered Lake Superior water

OTHER TEST CONDITIONS
- Photoperiod: 16 h
- Light intensity: 18-28 lumens

Nominal and measured concentrations:

7.3, 38.4, and 40.0 µg/l

Reference substance (positive control):

no

Details on estimation of bioconcentration:

At the end of the study whole fish were analyzed for residues using the methods described by Veith et al (1971).

Conc. / dose:

7.3 µg/L

Temp.:

>= 23 - <= 27 °C

pH:

7.5

Type:

BCF

Value:

< 11 dimensionless

Basis:

whole body w.w.

Conc. / dose:

38.4 µg/L

Temp.:

>= 23 - <= 27 °C

pH:

7.5

Type:

BCF

Value:

< 11 dimensionless

Basis:

whole body w.w.

Key result

Conc. / dose:

40 µg/L

Temp.:

>= 23 - <= 27 °C

pH:

7.5

Type:

BCF

Value:

< 11 dimensionless

Basis:

whole body w.w.

Details on results:

- Mortality of test organisms: LC50 (30 days) = 6.7 µg/l

Residues of the test substance in 30 day-old fathead minnows were less than 0.1 µg/g in all tanks. As a result the BCF was < 11 for all concentrations.

Validity criteria fulfilled:

not specified

Conclusions:

Residues of the test substance in 30 day-old fathead minnows were less than 0.1 µg/g in all tanks. As a result the BCF was < 11 for all concentrations.

Executive summary:

The bioaccumulation of hexachlorocyclopentadiene to fish was determined using a method similar to the OECD Guideline for Testing of Chemicals 305 (non GLP) with deviations. The absence of the depuration phase is not considered as significant considering that the BCF is less than 11.

The study was carried out on Pimephales promelas. The animals were exposed to concentrations of 7.3, 38.4, or 40.0 µg/l for 30 days under flow-through conditions. At the end of the study, they were analysed for residues of the substance.

Residues of the test substance in 30 day-old fathead minnows were less than 0.1 µg/g in all tanks. As a result the BCF was < 11 for all concentrations.

Description of key information

The bioaccumulation of Hexachlorocyclopentadiene in fish was determined using a method similar to the OECD Guideline for Testing of Chemicals 305 (non GLP). The absence of the depuration phase was not considered as significant considering that the BCF was less than 11.

The study was carried out onPimephales promelas (fathead minnow). The animals were exposed to concentrations of 7.3, 38.4 or 40.0 µg/l for 30 days under flow-through conditions. At the end of the study, they were analysed for residues of the substance.

Residues of the test substance in 30 day-old fathead minnows were less than 0.1 µg/g in all tanks. As a result the BCF was < 11 for all tested concentrations.

Key value for chemical safety assessment

BCF (aquatic species):

11 dimensionless