2-[N-(2-cyanoethyl)-4-[(2,6-dichloro-4-nitrophenyl)azo]anilino]ethyl acetate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

From September 10, 1997 to October 08, 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

abstract

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

GLP compliance:

no

Inoculum or test system:

other: sludge

Remarks:

100 mg/L suspended solids

Details on inoculum:

Waste water plan: ARA - PRO RHENO Basel communal

Duration of test (contact time):

28 d

Initial conc.:

30 mg/L

Based on:

ThOD

Remarks:

ThOD: 1.21 mg O2/mg

Parameter followed for biodegradation estimation:

other: biochemical oxygen demand

Details on study design:

An automated closed-system oxygen consumption measuring apparatus (BOD-meter) was used in this experiment.

Reference substance:

other: not specified

Key result

Parameter:

other: mean biodegradation (%)

Value:

6

Sampling time:

28 d

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Remarks on result:

other: after 5 d

Results with reference substance:

Biodegradation of the reference substance after 14 d: 80%

Validity criteria fulfilled:

not specified

Interpretation of results:

not inherently biodegradable

Conclusions:

Under the study conditions, the test substance biodegradation was equivalent to 6% after 28 d. The test substance was therefore not considered to be inherently biodegradable.

Executive summary:

A study was conducted to determine the inherent biodegradability of the test substance according to OECD Guideline 302 C. Sludge (100 mg/L suspended solids) from a predominantly domestic wastewater treatment plant was exposed for 28 d to the test substance at a concentration of 30 mg/L. The theoretical oxygen demand (ThOD) was 1.21 mg 02/mg. The test setup also included a standard reference substance. The biodegradation of the reference substance after 14 d was determined to be 80%. Under the study conditions, the test substance biodegradation was equivalent to 6% after 28 d. The test substance was therefore not considered to be inherently biodegradable (Dietschy, 1997).

Reference 2

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

From July 22, 1992 to August 08, 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Remarks:

1020 mg/L suspended solids

Details on inoculum:

From ARA Rhein Industrie

Duration of test (contact time):

28 d

Initial conc.:

398 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

TOC removal

Remarks:

%

Reference substance:

diethylene glycol

Remarks:

153.6 mg/L

Key result

Parameter:

% degradation (TOC removal)

Value:

33.1

Sampling time:

28 d

Details on results:

DOC (%): 37.7

Results with reference substance:

diethylene glycol (14 d biodegradation): 100%

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

Under the study conditions, the test substance biodegradation was equivalent to be 33.1%. The test substance was therefore not considered to be inherent biodegradable.

Executive summary:

A study was conducted to determine the inherent biodegradability of the test substance according to OECD Guideline 302 B. Sludge (1020 mg/L suspended solids) from a predominantly domestic wastewater treatment plant was exposed for 28 d to the test substance at a concentration of 398 mg/L (corresponding to 120.6 mg a.i./L). The test setup also included a reference substance, diethylene glycol at 153.6 mg/L. Degradation of the test substance in terms of % TOC was monitored for 28 d. The biodegradation of the reference substance after 14 d was determined to be 100%. Under the study conditions, the test substance biodegradation was equivalent to 33.1%. The test substance was therefore not considered to be inherent biodegradable (Dietschy, 1992).

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

not specified

Inoculum or test system:

not specified

Duration of test (contact time):

28 d

Initial conc.:

6 mg/L

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

5

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

Results with reference substance:

Aniline (28 d, ready biodegradability %): 78

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under the study conditions, the test substance biodegradation was equivalent to 5% after 28 d. The test substance was therefore not considered to be readily biodegradable.

Executive summary:

A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301 D. The inoculum (6 mg/L) was exposed for 28 d to the test substance. The oxygen demand was monitored for 28 d. The test setup also included the reference substance aniline. The biodegradation of the reference substance after 14 d was determined to be 75%. Under the study conditions, the test substance biodegradation was equivalent to 5% after 28 d. The test substance was therefore not considered to be readily biodegradable (Mueller, 1993).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

A study was conducted to determine the ready biodegradability of the test substance according to OECD Guideline 301 D. The inoculum (6 mg/L) was exposed for 28 d to the test substance. The oxygen demand was monitored for 28 d. The test setup also included the reference substance aniline. The biodegradation of the reference substance after 14 d was determined to be 75%. Under the study conditions, the test substance biodegradation was equivalent to 5% after 28 d. The test substance was therefore not considered to be readily biodegradable (Mueller, 1993).

A study was conducted to determine the inherent biodegradability of the test substance according to OECD Guideline 302 C. Sludge (100 mg/L suspended solids) from a predominantly domestic wastewater treatment plant was exposed for 28 d to the test substance at a concentration of 30 mg/L. The theoretical oxygen demand (ThOD) was 1.21 mg 02/mg. The test setup also included a standard reference substance. The biodegradation of the reference substance after 14 d was determined to be 80%. Under the study conditions, the test substance biodegradation was equivalent to 6% after 28 d. The test substance was therefore not considered to be inherently biodegradable (Dietschy, 1997).

A study was conducted to determine the inherent biodegradability of the test substance according to OECD Guideline 302 B. Sludge (1020 mg/L suspended solids) from a predominantly domestic wastewater treatment plant was exposed for 28 d to the test substance at a concentration of 398 mg/L (corresponding to 120.6 mg a.i./L). The test setup also included a reference substance, diethylene glycol at 153.6 mg/L. Degradation of the test substance in terms of % TOC was monitored for 28 d. The biodegradation of the reference substance after 14 d was determined to be 100%. Under the study conditions, the test substance biodegradation was equivalent to 33.1%. The test substance was therefore not considered to be inherent biodegradable (Dietschy, 1992).