1-chloro-2-nitrobenzene

Long-term toxicity to fish

Administrative data

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well-documented study, comparable to guideline. Adopted according to OECD SIDS. The original source is available and has been reviewed.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Subchronic toxicities of|industrial and agricultural chemicals to Fathead Minnows|(Pimephales promelas) Vol. I

Author:

Call, D.J. and Geiger, D.L.

Year:

1992

Bibliographic source:

Center for Lake Superior|Environmental Studies, Lake Superior Research Institute,|University of Wisconsin-Superior, USA

Reference 2

Reference Type:

secondary source

Title:

1-Chloro-2-nitrobenzene CAS: 88-73-3, SIDS Initial Assessment Report

Author:

OECD

Year:

2001

Bibliographic source:

UNEP publications

Materials and methods

Principles of method if other than guideline:

Method: other: comp. to OECD 210 (Fish, Early-life Stage Toxicity Test, 1992)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

IUCLID4 Test substance: other TS: 99%
- Name of test material (as cited in study report): 1-Chloro-2-nitrobenzene
- Origin: Aldrich Chemical Company

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Pimephales promelas

Study design

Test type:

flow-through

Test conditions

Hardness:

54.35 mg/L CaCO3

Test temperature:

24.81 °C (mean)

pH:

7.42 (mean)

Dissolved oxygen:

6.32 mg/L

Details on test conditions:

Photoperiod: 16 h light / 8 h dark
Total alkalinity, mean: 45.09 mg/L CaCO3

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

0.264 mg/L

Basis for effect:

larval development

Remarks on result:

other: % normal at hatch

Effect concentrations 2

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

2.07 mg/L

Basis for effect:

mortality

Remarks on result:

other: % survival

Effect concentrations 3

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

1.03 mg/L

Basis for effect:

weight

Effect concentrations 4

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

0.534 mg/L

Basis for effect:

length

Any other information on results incl. tables

OECD SIDS (2001):

In a first step 50 embryos were tested on hatchability and development after 4 - 5 days of incubation. In a second step 15 randomly selected frys from the initial egg cups where observed on their further development for 33 days. The 33 d-NOEC was determined by the authors Call & Geiger (1992) to be 0.264 mg/L based on the endpoint 'normal larvae' related to the hatched larvae. The review of the raw data of the study shows, that at the next higher test concentration of 0.530 mg/L a statistically significant effect compared to the control could be observed, however, there is no dose-effect relation for this endpoint at higher test concentrations. The highest test concentration of 3.9 mg/L shows less normal larvae after hatch with a deviation of 7% compared to the control. Apart from that regarding the endpoint 'normal larvae related to initial embryos' no effect at any concentration can be seen. Regarding 'weight' and 'length' of the fry, at both endpoints a deviation to the control of > 5% can be seen at a concentration of 2.04 mg/L. Also for this endpoint there is no dose-effect relationship seen at the next higher concentration. As statistically significant effects for the endpoint "normal larvae" were seen at concentrations above 0.264 mg/L, the NOEC derived by the authors is used for the hazard assessment for reasons of precaution.