1-chlorooctane

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

(proposed April 1997)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples from one test vessel of each concentration group were taken during the test: during the test solution preperation (day 0, 7, and 14) and prior to renewal (day 2, 9 and 16).

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: To obtain a stock solution, 100 mg test substance was dissolved per liter dilution water (with supplement HCO-50). The test concentrations were adjusted by addition of the required amount of stock solution to the dilution water. The test solutions were prepared freshly with every renewal.
- Controls: control with dilution water only; vehicle control with HCO-50 100 mg/L
- Chemical name of vehicle (: HCO-50 (1.00 wt%) served as supplement
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no precipitations were observed

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: National Institute for Enviromental Studies, Japan
- Feeding during test: yes
- Food type: Chlorella regularis
- Amount: 0.1 to 0.2 mg organic carbon/day/daphnia
- Frequency:

ACCLIMATION
- Acclimation period: 21 d
- Acclimation conditions (same as test or not): same as test

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

218 - 231 mg/L CaCO3

Test temperature:

19.1 - 20.6 °C

pH:

pH= 7.4 - 8.2

Dissolved oxygen:

7.4 - 8.8 mg/L

Nominal and measured concentrations:

nomial: 0.07 mg/L, 0.12 mg/L, 0.21 mg/L, 0.38 mg/L, 0.69 mg/L, 1.24 mg/L
measured: 0.033 - 0.85 mg/L
Recovery: 41- 76%

Details on test conditions:

TEST SYSTEM
- Test vessel: glass test vessel (100 mL)
- Material, size, headspace, fill volume: glass test vessel (100 mL) filled with 80 mL test solution
- Renewal rate of test solution (frequency/flow rate): semi-static, 3 times/week renewal of the test solution
- No. of organisms per vessel: 1/vessel, 10/concentration
- No. of vessels per concentration (replicates): 10 test vessels/ concentration

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
- Light intensity: Room light

VEHICLE CONTROL PERFORMED: yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

0.25 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

of the parents

Remarks on result:

other: 95% confidential interval: 0.20 - 0.40 mg/L

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.11 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

inhibition concentration

Remarks on result:

other: 95% confidential limit: 0.10 - 0.12 mg/L

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.073 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.17 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

- Mortality of parent animals: no mortality in the control and in group up to 0.073 mg/L. 70% mortality in the group with the highest concentration
- No. of offspring produced per day per female: control: 123 juveniles, vehicle control: 103 juveniles, lowest concentration 104, highest concentration: no juveniles
- Time to first brood release: control group: 8-9 d, vehicle control: 8-12 d, lowest concentration: 8-12 d, highest concentration: no offsprings

The EC50 was determined as 0.25 mg/L with a confidence limit of 0.10 to 0.12 mg/L. The NOEC was 0.073 mg/L, whereas the LOEC was 0.17 mg/L. The mortality of the parents is LC50= 0.25 mg/L with a confidence range of 0.2 to 0.4 mg/L.

Results with reference substance (positive control):

Reference substance potassium dichromate EiC50= 0.90 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for n-octylchlorid was determined as 0.25 mg/L with a confidence limit of 0.10 to 0.12 mg/L. The NOEC was 0.073 mg/L, whereas the LOEC was 0.17 mg/L. The mortality of the parents is LC50= 0.25 mg/L with a confidence range of 0.2 to 0.4 mg/L.