1-chlorooctane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

(1984)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorooctane

EC Number:

203-915-5

EC Name:

1-chlorooctane

Cas Number:

111-85-3

Molecular formula:

C8H17Cl

IUPAC Name:

1-chlorooctane

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

A sample of the test solution was taken from each vessel at the start of the test and after 24 h.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: addition of 500 mg test substance per liter dilution water
- Controls: vehicle control with 100 mg/L vehicle
- Chemical name of vehicle: HCO-50
- Concentration of vehicle in test medium: 100 mg/L
- Evidence of undissolved material: no precipitation observed.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna younger than 24 hours were used.
The animals were derived from mature Daphnia but not the first brood progeny. Dormant eggs or males were not observed. The mortality of the population was low.

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: younger than 24 h
- Method of breeding: in dilution water (elendt M4 according to guideline OECD 211). 20-40 Daphnia/L breeding water
- Feeding during test: no
- Food type: Chlorella regularis
- Amount: 0.1-0.2 mg organic carbon/day/Daphnia

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

water renewed every 24 h

Test conditions

Hardness:

242 mg/L CaCO3 in dilution water

Test temperature:

20±1 °C

pH:

dilution water: 8.1; during the test: 7.7 - 7.9

Dissolved oxygen:

7.5 mg/L - 8.5 mg/L

Nominal and measured concentrations:

nominal: 0.5 mg/L, 0.9 mg/L, 1.7 mg/L, 3.1 mg/L, 5.6 mg/L, 10 mg/L
measured concentrations: beginning of the test: 0.19 - 6.28 mg/L; after 24 h: <0.08 - 1.82 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass vessels
- Material, size, headspace, fill volume: glass, 100 mL test volume
- Type of flow-through: semi-static
- Renewal of test solution: every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Light intensity: 16 h light, 8h dark

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 (according to OECD 211)
- Ca/mg ratio: 242 mg/L CaCO3
-pH: 8.1

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.22 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95%: 0.16 mg/L - 0.28 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.21 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.16 mg/L - 0.28 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.16 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- The EC50 of the test substance was determined as 0.21 mg/L after 48 h with a confidence range of 0.16 mg/L - 0.28 mg/L. The NOEC of the test substance is 0.16 mg/L after 48 h.
- Mortality of control: none
- Any observations: An examination of the test solutions before the test showed no precipiations.

Results with reference substance (positive control):

EiC50 of potassium dichromate was 0.84 mg/L after 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the test substance was determined as 0.21 mg/L after 48 h with a confidence range of 0.16 mg/L - 0.28 mg/L. The NOEC of the test substance is 0.16 mg/L after 48 h.