Registration Dossier
Registration Dossier
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EC number: 202-455-2 | CAS number: 95-82-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: DNA damage and/or repair
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The genotoxicity of a variety of aniline derivates in a DNA repair test with primary cultured rat hepatocytes
- Author:
- Yoshimi N., Sugie S., Iwata H., Niwa K., Mori H., Hashida Ch., Shimizu H.,
- Year:
- 1 987
- Bibliographic source:
- Mutation Research, vol. 206, p. 183-191, 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Williams G.M., Laspia M.F., Dumkel V.C., Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens, Mutation Research, vol. 97, p. 359-370, 1982.
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of assay:
- DNA damage and repair assay, unscheduled DNA synthesis in mammalian cells in vitro
Test material
- Reference substance name:
- 2,5-dichloroaniline
- EC Number:
- 202-455-2
- EC Name:
- 2,5-dichloroaniline
- Cas Number:
- 95-82-9
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,5-dichloroaniline
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- hepatocytes:
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- doses 1, 0.1, 0.01 and 0.001 mmol of the test substance
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
Results and discussion
Test results
- Species / strain:
- hepatocytes:
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: rat hepatocytes
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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