2,5-dichloroaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study without detailed description

Data source

Reference

Reference Type:

publication

Title:

QSAR study on the toxicity of substitued benzenes to the algae (Scenedesmus obliquus)

Author:

Lu G.H., Yuan X., Zhao Y.H.

Year:

2001

Bibliographic source:

Chemosphere, vol. 44, p. 437-440, 2001

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,5-dichloroaniline

EC Number:

202-455-2

EC Name:

2,5-dichloroaniline

Cas Number:

95-82-9

Molecular formula:

C6H5Cl2N

IUPAC Name:

2,5-dichloroaniline

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

other: Scenedesmus obliquus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20±1°C

pH:

7.2±0.2

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml Erlenmeyer flasks (fill in 60 ml)
- Initial cells density: cca 10000 cells/ml


GROWTH MEDIUM
- Detailed composition if non-standard medium was used:
NH4Cl 15 mg/l
MgCl2 . 6H2O 12 mg/l
CaCl2 . 2H2O 18 mg/l
MgSO4 . 7H2O 15 mg/l
KH2PO4 1.6 mg/l
NaHCO3 50 mg/l
Na2EDTA . 2H2O 0.1 mg/l
FeCl3 . 6H2O 0.08 mg/l
H3BO3 0.185 mg/l
MnCl2 . 4H2O 0.415 mg/l
ZnCl2 0.003 mg/l
CoCl2 . 6H2O 0.0015 mg/l
Na2MoO4 . 2H2O 0.007 mg/l
CuCl2 . 2H2O 0.00001 mg/l


OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity and quality: cca 400 lux (white fluorescent lamp)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electron microscope (400×)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.15 mol/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified