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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study without detailed description

Data source

Reference
Reference Type:
publication
Title:
QSAR study on the toxicity of substitued benzenes to the algae (Scenedesmus obliquus)
Author:
Lu G.H., Yuan X., Zhao Y.H.
Year:
2001
Bibliographic source:
Chemosphere, vol. 44, p. 437-440, 2001

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-dichloroaniline
EC Number:
202-455-2
EC Name:
2,5-dichloroaniline
Cas Number:
95-82-9
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,5-dichloroaniline

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
other: Scenedesmus obliquus

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20±1°C
pH:
7.2±0.2
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 ml Erlenmeyer flasks (fill in 60 ml)
- Initial cells density: cca 10000 cells/ml


GROWTH MEDIUM
- Detailed composition if non-standard medium was used:
NH4Cl 15 mg/l
MgCl2 . 6H2O 12 mg/l
CaCl2 . 2H2O 18 mg/l
MgSO4 . 7H2O 15 mg/l
KH2PO4 1.6 mg/l
NaHCO3 50 mg/l
Na2EDTA . 2H2O 0.1 mg/l
FeCl3 . 6H2O 0.08 mg/l
H3BO3 0.185 mg/l
MnCl2 . 4H2O 0.415 mg/l
ZnCl2 0.003 mg/l
CoCl2 . 6H2O 0.0015 mg/l
Na2MoO4 . 2H2O 0.007 mg/l
CuCl2 . 2H2O 0.00001 mg/l


OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity and quality: cca 400 lux (white fluorescent lamp)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electron microscope (400×)
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.15 mol/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified