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Diss Factsheets

Administrative data

Endpoint:
nanomaterial aspect ratio/shape
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2020
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
other: unpublished study report
Title:
Unnamed
Year:
2020
Report date:
2020

Materials and methods

Principles of method if other than guideline:
Guidelines from NanoDefine are applied:
Method selection according to Gaillard et al., Nanoscale Adv., 2019, 1, 781
TEM image evaluation according to Verleysen et al., Materials 2019, 12, 2274; doi:10.3390/ma12142274
GLP compliance:
no
Type of method:
transmission electron microscopy
Details on methods and data evaluation:
Sample Preparation: Dispersion in ethanol; transfer to carbon-coated TEM-grids.

Test material

Constituent 1
Chemical structure
Reference substance name:
4,11-dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
EC Number:
221-423-9
EC Name:
4,11-dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
Cas Number:
3089-16-5
Molecular formula:
C20H10Cl2N2O2
IUPAC Name:
4,11-dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
Test material form:
solid: nanoform
Details on test material:
- Name of test material (as cited in study report): 4,11-Dichloroquinacridon
- Analytical purity: 98.4% (w/w)
- Lot/batch No.: 200389

Data gathering

Instruments:
Thermo-Fisher Osiris 200 kV

Calibration:
no data
Reproducibility:
no data

Results and discussion

Shape description
Key result
Shape:
other: orthorhombic
Remarks on result:
other: The material is assigned to the “multimodal” shape category.
Aspect ratio
Key result
x Dimension:
1.8
St. dev.:
1.7
y Dimension:
1
Remarks on result:
other: median (D50)

Any other information on results incl. tables

The number-based concentration of particles with sizes below 100 nm (smallest feret diameter) is 99%. The median (D50) of the particle size is 43,3 nm.

Applicant's summary and conclusion

Conclusions:
- The particles have a median aspect ratio of 1,8:1
- The shape is “orthorhombic”. The material is assigned to the “multimodal” shape category.
- The number-based concentration of particles with sizes below 100 nm (smallest feret diameter) is 99%. The median (D50) of the particle size is 43,3 nm.
The material is therefore classified as “nano” according to the definition of nanomaterial 2011/696/EU.