1-ethyl-1-methylpyrrolidinium bromide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 1991- December 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

CAS No: 69227-51-6
Batch No: 0691
pH: 6-9
Storage: 5°C in the dark
Supplier: Chemson, Polymer-Additive Gesellschaft m.b.H A-9601 Arnoldstein
Appearance: Colorless liquid

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 52.2%
- Sampling method: mercumetric titration of bromide
- Sample storage conditions before analysis: 5°C in the dark

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: all solutions for the test were prepared by diluting of stock solutions with dilution water to 300 ml for each concentration immediately before the start of the test.
Test substance stock solution: 960 mg test substance dissolved in 800 ml dilution water (concentration: 1200 mg/l)
Reference substance stock solution: 50 mg potassium dichromate dissolved in 500ml dilution water and further diluted with dilution water (final concentration of the stock solution: 10 mg/l)

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Institute fur wassergute und Landschaftswasswrbau, Technische Universitat Wien, A-1040 Wien.
- Age of parental stock (mean and range, SD): Between 6 and 24 hr at the start of the test
- Feeding during test: no feeding

ACCLIMATION
- Acclimation period: the Daphnia were used for the test immediately after receipt

Test type:

static

Water media type:

other: Synthetic water, obtained from the supplier of the Daphnia prepared according to DIN 38412.

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

N/A

Post exposure observation period:

N/A

Hardness:

N/A

Test temperature:

between 20.0-20.5 °C controlled by laboratory thermometer

pH:

8.3 negative control (start of experiment), 8 (after 48 hr)
8.1 and 8.2 test material (start of experiment), 80 and 8.1 after 48 hr

Dissolved oxygen:

99.1-117.2

Nominal and measured concentrations:

0, 200, 313, 490, 767, 1200 mg/l (nominal) test substance
0.20, 0.41, 0.84, 1.71, 3.50 mg/l (nominal) control (potassium dichromate)

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- No. of organisms per vessel:10 daphnia per group, 5 daphnia for highest concentration (preliminary test), 5 Daphnia per beaker, 4 concenrrations (0.1, 1, 10, 100 and 1000 mg test substance per liter water and one negative control group (dilution water), exposure time 48 hr.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic water, obtained from the supplier of the daphnia prepared according to DIN 38412, aerated before use.

OTHER TEST CONDITIONS
- Adjustment of pH: Determined with Ph meter (G103, Seibold, A-1010 Vienna)
- Photoperiod: Complete darkness

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

660 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

All animals were observed for alternations and immobilisation immediately after the start of the exposure and 24 and 48 hr thereafter. Daphnia was considered immobile when they were not able to swim within 15 seconds after gentle agitation of the beaker.

EC50/24 hr > 1200 mg/l
EC 50/24 hr 660 mg/l (Key data)
EC50/48 hr the max concentration of the test substance causing no immobilisation within the period of the test was 200 mg/l
EC100/48 hr >1200 mg/l

Results with reference substance (positive control):

The EC/24h of the reference substance was 1.48mg pottasium dichromate per liter water (95% confidence limits: 1.19 and 1.85 mg/l), the EC50/48 h was 0.90 mg/l (95% confidance limits: 0.77 and 1.06 mg/l).

Reported statistics and error estimates:

EC50 values and confidence limits were determined by method of W. Thompson (Bact. Rev. 11, 1947) These calculations were based on the number of allDaphnia of each relevant concentration (group).

Validity criteria fulfilled:

yes

Conclusions:

EC 50/ 48 hr = 660 mg/l 50 % Aqueous solution of N-Ethyl-N-Methyl-Pyrollidinium-Bromid (95% confidence limits: 560 and 770 mg/l)

Executive summary:

The acute toxicity of 50 % Aqueous solution of N-Ethyl-N-Methyl-Pyrollidinium-Bromid was determined in the fresh water crustacean Daphnia magna over test period of 48 hr (static test). Concentrations of the test substance in dilution water were 200, 313, 490, 767 and 1200 mg/l. 20 Daphnia were used for each concentration and one for negative control (dilution water, untreated) (OECD 202).

To check the reliability of the test conditions potassium dichromate was used as reference substance (five concentrations between 0.20 and 3.50 mg/l, 20 Daphnia per concentration).

Investigation performed: pH, temperature, dissolved oxygen concentrations; number of immobile Daphnia after 24 and 48 hr.

The OECD criteria for the validity of the test was fulfiled.

No control Daphnia were immobilised or trapped at the surface of the water over the test period.

The dissolved oxygen concentration at the end of the test was distinctly greater than 60% of the air saturation value in all tested concentrations.

The pH of the test substance was comparable to the pH of the negative control at the start and at the end of the test.

Results:

EC 50/ 48 hr = 660 mg/l  50 % Aqueous solution of N-Ethyl-N-Methyl-Pyrollidinium-Bromid (95% confidence limits: 560 and 770 mg/l) (Key data)

EC50/24 hr > 1200 mg/l, EC50/48 hr the max concentration of the test substance causing no immobilisation within the period of the test was 200 mg/l

EC100/48 hr  >1200 mg/l

Description of key information

EC 50/ 48 hr = 660 mg/l  50 % Aqueous solution of N-Ethyl-N-Methyl-Pyrollidinium-Bromid (95% confidence limits: 560 and 770 mg/l)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

660 mg/L