1,3,3-trimethyl-2-[[methyl(p-tolyl)hydrazono]methyl]-3H-indolium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read across from similar substance

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Sufficiently well documented study according to national guideline.

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

- Preparation of a stock solution of 120 mg/L in water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Daphnia magna Strauss- Strain: not available- Source: Bayer AG Leverkusen- Age at study initiation (mean and range, SD): 6-24 hrs- Food type: Scenedesmus Subspicatus (ad libitum)- Frequency: Daily- Health during acclimation (any mortality observed): none- Cultivation: daily feeding with Scenedesmus Subspicatus (ad libitum) (SOP 4003)- Water : "aufbereitetes Leverkusener Trinkwasser" of drinking quality.- Medical pre-treatment: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Nominal and measured concentrations:

Nominal test concentrations: 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L

Details on test conditions:

- Test vessels (cylindrical) : 4 cm diameter and 7 cm high- Volume of the test medium: 20 mL- Ventilation: none- Reference substance: Potassium dichromate- Measurement and numerical values during the test: Oxygen, pH, Temperature of water, Number of immobile Daphnia

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.57 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

- Results with reference substance valid? yes

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (24 h) of the substance to Daphnia magna was determined to be 0.57 mg/L.

Executive summary:

The 24–hr-acute toxicity of the test item to Daphnia magna was studied at nominal concentrations of 0.32, 1.0, 3.2, 10, 31.6 and 100 mg/L. Test conditions were similar to the standard test guideline EU Method C.2 (Acute toxicity for Daphnia).

The 24– hour EC₅₀was determined to be 0.57 mg/L. 

A classification for aquatic toxicity based on the results of this study is not possible as the test duration is too short (24hr instead of 48hr).

Description of key information

EC50 = 0.57 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.57 mg/L

Additional information

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).
Based on the results, the classification in Aquatic Acute and Chronic 1 is required by CLP Regulation.