Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers

EC Number:

249-236-8

EC Name:

Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers

Cas Number:

28804-46-8

Molecular formula:

C16H14Cl2

IUPAC Name:

Reaction product of tricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene with chlorine

Test material form:

solid: granular

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

Before conducting the preliminary range finding study, test item was checked for solubility and precipitation. Test item was found to be insoluble in RO water at the concentration of 0.1 mg/mL. Further solubility was checked in dimethyl formamide (DMF), acetone and Dimethyl sulfoxide (DMSO) and found to be soluble at 33.33 (Stock A), 28.57 (Stock B) and 22.22 (Stock C) mg/mL, respectively. Maximum solubility of test item found in dimethyl formamide (DMF) and acetone. Hence, test item soluble in DMF and acetone was further serially diluted in respective solvents and checked for precipitation in the test medium.
Stock solution A was serially diluted at the concentration of 16.66 (Stock D), 8.33 (Stock E), 4.16 (Stock F), and 2.08 (Stock G) mg/mL in DMF. These stock solutions were checked for precipitation test using RO water. Precipitation was observed upto the concentration of 4.16 mg/mL, while no precipitation was observed at the concentrations of 2.08 mg/mL.
Stock solution B was serially diluted at the concentration of 14.28 (Stock H), 7.14 (Stock I) and 3.57 (Stock J) mg/mL in acetone. These stock solutions were checked for precipitation test using RO water. Precipitation was observed upto the concentration of 7.14 mg/mL, while no precipitation was observed at the concentrations of 3.57 mg/mL.
Hence, the Acetone was used as vehicle for the study. And the highest tested concentration was used 0.357 mg/L.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Remarks:

limit test

Effect conc.:

> 0.357 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study was conducted as a limit test in which the 96 h LC50 of DPX-C (Di-Cloro-Di-p-Xililene) was found to be greater than 0.357 mg/L.

Executive summary:

This study was performed to assess the acute toxicity (96 h) of DPX-C (Di-Cloro-Di-p-Xililene) to the Rainbow Trout according to the OECD guideline 203.

Based on the results of the preliminary range finding study, thirty fish individuals were divided into three groups of ten.The study was conducted as a limit study under a semi-static conditions in which one group of fish was exposed to a limit concentration of 0.357 mg/L and one group served as control second group served as vehicle control.

The stability of test item in test media was also performed during method validation (JRF StudyN°228-2-13-14897) and test item in test media found to be stable up to 24 h (> 80% of nominal concentration). Test media was analysed forDPX-C (Di-Cloro-Di-p-Xililene)active ingredient concentration and stability to monitor the concentration of test solution at 0 and 24 h of first renewal during main study. The active ingredient concentration of test item in test media was within acceptable limit (> 80% of nominal concentration). 

The study was conducted as a limit test in which the 96 h LC₅₀ofDPX-C (Di-Cloro-Di-p-Xililene) wasfound to be greater than 0.357mg/L.