Isopropyl 4-hydroxybenzoate

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Chronic toxicity test was performed for 7 days at different concentrations.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Isopropylparaben
- Molecular formula: C10-H12-O3
- Molecular weight: 180.202 g/mol
- Smiles notation: c1(C(OC(C) C)=O)ccc(O)cc1
- InChl : 1S/C10H12O3/c17( 2)1310(12)8359(11)648/h37,11H,12H3
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Age at study initiation (mean and range, SD): 24 h posthatch larvae
- Feeding during test: yes
- Food type: newly hatched Artemia nauplii
- Frequency: twice daily

Test type:

static

Water media type:

freshwater

Total exposure duration:

7 d

Hardness:

160 to 175 mg/L as CaCO3

Test temperature:

24°C

pH:

8.2 to 8.8

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentration: Five concentrations

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 250 ml
- Aeration: Yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): four replicates

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test solution + a RHW and 0.1% acetone solvent control
- Alkalinity: 110 to 120 mg/L as CaCO3
- Conductivity: 535 to 588 mS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: 8.2 to 8.8
- Photoperiod: 16:8 light: dark cycle

TEST CONCENTRATIONS
- Range finding study: Half of the LC50 determined from the acute test was used as the highest subchronic exposure concentration.
- Test concentrations: five concentration was mention but values are not specified (range from 1.0 to 25 mg/L)

Reference substance (positive control):

not specified

Key result

Duration:

7 d

Dose descriptor:

LOEC

Effect conc.:

9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

not specified

Conclusions:

In long term toxicity study the low observed effect (LOEC) concentration for test species Pimephales promelas was observed to be 9.0 mg/L concentrations for Isopropylparaben(4191-73-5).

Executive summary:

Long term toxicity to fish was performed for 7 days at five different concentrations ranges from 1.0 to 25 mg/L. Four replicates of 10 P. promelas in 250 ml of test solution for each concentration were prepared and renewed daily. Fish were fed twice daily and mortality was observed daily. After 7 d of exposure, average weight per fish was calculated and compared to the control.

Thus, on the basis of above result the LOEC for long term toxicity to P. promelas was observed to be 9.0 mg/L for test chemical Isopropylparaben (4191-73-5)

Description of key information

In long term toxicity study the low observed effect (LOEC) concentration for test species Pimephales promelas was observed to be 9.0 mg/L concentrations for Isopropylparaben(4191-73-5).

Additional information

Long term toxicity to fish was performed for 7 days at five different concentrations ranges from 1.0 to 25 mg/L. Four replicates of 10 P. promelas in 250 ml of test solution for each concentration were prepared and renewed daily. Fish were fed twice daily and mortality was observed daily. After 7 d of exposure, average weight per fish was calculated and compared to the control.

Thus, on the basis of above result the LOEC for long term toxicity to P. promelas was observed to be 9.0 mg/L for test chemical Isopropylparaben (4191-73-5)