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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study was conducted before GLP and guidelines and thus report detail is lacking. However, the study was done at a reputable laboratory to the state of the art at the time.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1957

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Method is similar to an OECD 402. Test subjects had test article applied to the skin under an occlusive dressing for 24 hours followed by a 14-day observation period.
GLP compliance:
no
Test type:
other:
Limit test:
no

Test material

Constituent 1
Reference substance name:
iso-Butyronitrile
IUPAC Name:
iso-Butyronitrile
Constituent 2
Chemical structure
Reference substance name:
Isobutyronitrile
EC Number:
201-147-5
EC Name:
Isobutyronitrile
Cas Number:
78-82-0
Molecular formula:
C4H7N
IUPAC Name:
2-methylpropanenitrile

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
None given

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: 90/10 Acetone/Corn oil
Details on dermal exposure:
Test article was applied neat under occlusive contitions to the test subjects.
Duration of exposure:
24 hours
Doses:
1) Undiluted (5 and 10 cc/kg)
2) 20% in 90/10 acetone/corn oil (5-20 cc/kg)
3) 5% in 90/10 acetone/corn oil (5-20 cc/kg)
Control animals:
no

Results and discussion

Effect levels
Key result
Dose descriptor:
LD50
Effect level:
< 20 other: cc/kg
Gross pathology:
Moderate edema, slight or no redness in lower doses. In highest dose, gross edema, moderate redness at edges, brown necrosis at edges.

Applicant's summary and conclusion

Interpretation of results:
very toxic
Remarks:
Migrated information Criteria used for interpretation of results: US CPSC / US OSHA
Conclusions:
The acute dermal LD50 was > 20 cc/kg as a 5% suspension.
Executive summary:

The acture dermal LD50 of iso-butyronitrile in guinea pigs was:

1) LD50 of < 5 cc/kg for undiluted test article.

2) < 5 cc/kg for 20% in 90/10 acetone/corn oil

3) > 20 cc/kg for 5% in 90/10 acetone/corn oil.