α-chloro-4-fluoroacetophenone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: The test follows the EU Directive 79/831/EEC, Annex V, part C, which is comparable to the OECD guideline 202.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

α-chloro-4-fluoroacetophenone

EC Number:

207-256-4

EC Name:

α-chloro-4-fluoroacetophenone

Cas Number:

456-04-2

Molecular formula:

C8H6ClFO

IUPAC Name:

2-chloro-1-(4-fluorophenyl)ethan-1-one

Details on test material:

- Name of test material (as cited in study report): p- Fluoro-w-chloroacetophenone
- Physical state: Solid
- Analytical purity: > 99%
- Lot/batch No.: 23473/86/2

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (24 h at 20 °C) . The nominal concentration of the stock solution was 100 mg/l.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source:Daphnia magna STRAUS, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation: 2 - 24 hours old

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 ± 0.5 mmol/L

Test temperature:

19.6 - 20.8 °C

pH:

7.9 - 8.3

Dissolved oxygen:

8.1 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 0.02, 0.04, 0.08, 0.16, 0.32, 0.63, 1.25, 2.5, 5 mg/L
Recovery rate (study initiation, unstocked): -, 120, n.m., n.m., n.m., n.m., 91, n.m., n.m., n.m., 88 %
Recovery rate (study end, unstocked): -, 60, n.m., n.m., n.m., n.m., 85, n.m., n.m., n.m., 99 %
Recovery rate (study end, stocked): -, -, n.m., n.m., n.m., n.m., 60, n.m., n.m., n.m., 80 %
(n.m. = not measured)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 ml glass tubes with flat bottom, 10 ml test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline;
M4 medium with the properties:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/L
Ratio Ca/Mg ions: about 4 : 1
pH value: 7.5 - 8.5
Conductivity: 600 - 700 microS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (about 2-7 microeinstein/m*m*s (400-750 nm))

EFFECT PARAMETERS MEASURED :
Observations: mobile daphnids after 24 and 48 h
Measurements: pH and oxygen content after 0 and 48 h; temperature: 0, 24, 48 h.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.3 - 0.5 CL

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility