1-chloro-4-nitrobenzene

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS; basic data given (comparable to guidelines)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Reference 2

Reference Type:

secondary source

Title:

1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.

Author:

OECD

Year:

2002

Bibliographic source:

UNEP Publications

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 202, part 2 (1984)

Deviations:

yes

Remarks:

; only four concentrations used; counting not made on a daily basis

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): p- chlornitrobenzol
- Analytical purity: 99.7%

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

around 20 °C

pH:

8.3 ± 1

Nominal and measured concentrations:

- The following TS concentrations were tested: 0.16, 0.5, 1.58 and 5.0 mg/l

Details on test conditions:

- Per concentration 10 trials were carried out in parallel starting with 1 Daphnia in each vessel
- Control consisted of 20 animals
- Test medium was renewed 3 times a week

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

0.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

0.103 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: based on an analytical recovery of 69.2 %

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.92 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.02 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: based on an analytical recovery of 69.2 %

Details on results:

According to OECD SIDS:
The lowest effect value was a 21d-EC10 of 0.103 mg/l for reproduction of Daphnia magna. This value was calculated from the
nominal EC10 of 0.15 mg/l using the analytical recovery of 69.2 % from the test concentration 1.5 mg/l.