1-chloro-4-nitrobenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Reference 2

Reference Type:

secondary source

Title:

1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.

Author:

OECD

Year:

2002

Bibliographic source:

UNEP Publications

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

; limit test not reported

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 1-chlor-4-nitrobenzene
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

- The stock solution of the test substance was prepared in aceton and finally diluted in water, so that a concentration of aceton of 0.2 ml/l
was present in the test.
- The test was conducted in 11 l aquarium filled with 10 l test solution.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Before starting the test fish was maintained at least 12 days in dilution water.
- Ten fishes were used per concentration.
- The mean fish length was 2.0 +/- 1.0 cm.
- Fishes were exposed to a cycle of 14 h light and 10 h darkness.

Study design

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

23 ± 1 °C

pH:

8.15 ± 0.2

Nominal and measured concentrations:

- For LC50 determination, the following concentrations were used: 0, 3.62, 6.8, 13.1, 24.6 and 50 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: nominal concentration

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

15 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: analytical concentration

Details on results:

Same values for 48 h

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
Furthermore the substance was tested according to the method OECD 204: "Fish, Prolonged toxicity test (14 days) under the same test conditions.
As endpoint the following sub-lethal effects were observed: feeding behaviour, respiration, weight and malposition.
The results of this test were: NOEC= 1.53 mg/l; LOEC=13.2 mg/l and NOLEC(no observed lethal effect concentration) = 3.03 mg/l