1,3-dichloro-4-nitrobenzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study but no GLP defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dichloro-4-nitrobenzene

EC Number:

210-248-3

EC Name:

1,3-dichloro-4-nitrobenzene

Cas Number:

611-06-3

Molecular formula:

C6H3Cl2NO2

IUPAC Name:

1,3-dichloro-4-nitrobenzene

Test material form:

other: solid

Details on test material:

IUCLID4 Test substance: as prescribed by 1.1 - 1.4

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: one eye of each rabbit served as control and remained untreated

Amount / concentration applied:

100 mg

Duration of treatment / exposure:

24 h

Observation period (in vivo):

7 d

Number of animals or in vitro replicates:

3

Details on study design:

24 hours affter application of test item into the conjunctival sac, the treated eye was rinsed with saline.

Results and discussion

In vivo

Results

Irritation parameter:

other: according to DRAIZE

Basis:

mean

Time point:

other: 24, 48 and 72 h

Score:

0

Max. score:

110

Reversibility:

fully reversible

Remarks on result:

other: not irritating

Other effects:

-

Any other information on results incl. tables

Reading	1 h			24 h			48 h			72
Animal No.	19	20	21	19	20	21	19	20	21	19	20	21
1. Cornea
a) cloudiness	0	0	0	0	0	0	0	0	0	0	0	0
b) surface	0	0	0	0	0	0	0	0	0	0	0	0
a=A*B*5	0	0	0	0	0	0	0	0	0	0	0	0
2. Iris	0	0	0	0	0	0	0	0	0	0	0	0
b=A*5	0	0	0	0	0	0	0	0	0	0	0	0
3.conjunctiva
a) reddening	1	1	1	0	0	0	0	0	0	0	0	0
b) swelling	1	1	1	0	0	0	0	0	0	0	0	0
c) defluxion	1	1	1	0	0	0	0	0	0	0	0	0
c=(A+B+C)*2	6	6	6	0	0	0	0	0	0	0	0	0
Total= a+b+c	6	6	6	0	0	0	0	0	0	0	0	0

Primary irritation value: 0/9= 0.0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Executive summary:

In an eye irritation study according to OECD 405 3 rabbits were treated with 0.1 g of the test substance. One eye of each test animal remained untreated and served as control. After 24 h the treated eye was flushed with with saline. The observation period was 7 d. According to DRAIZE the substance was found to be not irritating.