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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
None
Analytical monitoring:
yes
Details on sampling:
No data
Vehicle:
not specified
Details on test solutions:
No data
Test organisms (species):
Daphnia magna
Details on test organisms:
The study was performed with young daphnids of a clone of the species Daphnia magna Straus. The clone was originally supplied by the University of Sheffield/UK in 1992, defined from the supplier as clone 5. Since this date the clone is bred in the laboratories of RCC in reconstituted water of identical quality (regarding pH, main ions and total hardness) and under identical temperature and light conditions as in the tests. At the start of the test the used test organisms were 6-24 hours old and were not first brood progeny.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
No data
Hardness:
2.5 mmol/l (= 250.0 mg/l) as CaC03
Test temperature:
21 °C during the test period
pH:
7.8 to 8.0
Dissolved oxygen:
The dissolved oxygen concentrations in the test medium and the control were at least 8.1 mg/l.
Salinity:
No data
Nominal and measured concentrations:
Nominal concentration : 100mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:The beakers were covered with glass plates to reduce the loss of water and to avoid the entry of dust into the solutions.
- Aeration:The test water was aerated until oxygen saturation was reached. During the test period the test water was not aerated.

TEST MEDIUM / WATER PARAMETERS
- Alkalinity:0.8 mmol/l
- Ca/mg ratio:4:1 (based on molarity)
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A limit test was performed in accordance with the guidelines to demonstrate that the test item has no toxic effect on the test organisms up to and including the highest test item concentration which could be dissolved or very finely dispersed in test water.
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

None

Validity criteria fulfilled:
yes
Conclusions:
The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour ECO of FAT 60253/A to Daphnia magna were determined to be at least 0.010 mg/l (loading rate of 100 mg/l).
Executive summary:

The acute toxicity of the test item FAT 60253/A to Daphnia magna was determined in a 48-hour static test according to the Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals, No. 202, Part I (1984). A limit test was performed in accordance with the Commission Directive 92/69/EEC to demonstrate that the test item has no toxic effect on the test organisms up to and including the highest test item concentration which can be dissolved or very finely dispersed in test water. Due to the very low water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100 mg/l was continuously stirred at room temperature in the dark over 7 days. Then, the dispersion was filtered. Only the undiluted filtrate with the maximum concentration of dissolved and very finely dispersed test item was used as the test medium. Additionally, a control was tested in parallel. The analytically determined test item concentration in the test medium were 0.0093 and 0.011 mg/l at the start and the end of the test period, respectively. Under the test conditions, the test item was stable during the test period of 48 hours. All biological results are related to the mean measured test item concentration of 0.010 mg/l, calculated as the average of all measurements. In the control and in the undiluted filtrate, no immobilized or dead test organisms or other signs of intoxication were determined during the test period of 48 hours. The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour EC0 of FAT 60'253/A to Daphnia magna were determined to be at least 0.010 mg/l (loading rate of 100 mg/l). The 48-hour NOEC and the 48 -hour EC0 might even be higher but concentrations of the test item above the maximum concentration which could be dissolved or very finely dispersed in the test water were not tested, according to the guidelines. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the solubility limit of the test item in the test water. These values could not be quantified, since the test item had no toxic effect on the daphnids at the highest test item concentration which could be dissolved or very finely dispersed in the test water.

Description of key information

The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour ECO of FAT 60253/A to Daphnia magna were determined to be at least 0.010 mg/l (loading rate of 100 mg/l).

Key value for chemical safety assessment

Additional information

The acute toxicity of the test item FAT 60253/A to Daphnia magna was determined in a 48-hour static test according to the Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals, No. 202, Part I (1984). A limit test was performed in accordance with the Commission Directive 92/69/EEC to demonstrate that the test item has no toxic effect on the test organisms up to and including the highest test item concentration which can be dissolved or very finely dispersed in test water. Due to the very low water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100 mg/l was continuously stirred at room temperature in the dark over 7 days. Then, the dispersion was filtered. Only the undiluted filtrate with the maximum concentration of dissolved and very finely dispersed test item was used as the test medium. Additionally, a control was tested in parallel. The analytically determined test item concentration in the test medium were 0.0093 and 0.011 mg/l at the start and the end of the test period, respectively. Under the test conditions, the test item was stable during the test period of 48 hours. All biological results are related to the mean measured test item concentration of 0.010 mg/l, calculated as the average of all measurements. In the control and in the undiluted filtrate, no immobilized or dead test organisms or other signs of intoxication were determined during the test period of 48 hours. The 48-hour NOEC (highest concentration tested without toxic effects after the exposure period of 48 hours), and the 48-hour EC0 of FAT 60'253/A to Daphnia magna were determined to be at least 0.010 mg/l (loading rate of 100 mg/l). The 48-hour NOEC and the 48-hour EC0 might even be higher but concentrations of the test item above the maximum concentration which could be dissolved or very finely dispersed in the test water were not tested, according to the guidelines. The 48-hour EC50 and the 48-hour EC100 were clearly higher than the solubility limit of the test item in the test water. These values could not be quantified, since the test item had no toxic effect on the daphnids at the highest test item concentration which could be dissolved or very finely dispersed in the test water.