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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

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Description of key information

Biodegradation in water

42-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical (Experimental study report, 2019). The study was performed at a temperature of 20°C. The test system included control, test chemical and reference chemical. Polyseed were used as a test inoculum for the study.The concentration of test and reference chemical (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test chemical and reference chemical. The % degradation of procedure control (reference chemical) was also calculated using BOD & ThOD and was determined to be 66.26%. Degradation of Sodium Benzoate exceeds 70.48% on 7 days & 73.49% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD42 value of test chemical was observed to be 0.75 mgO2/mg. ThOD was calculated as 2.64 mgO2/mg. Accordingly, the % degradation of the test chemical after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 28.4%. Based on the results, the test item, under the test conditions, was considered to be inherently biodegradable in nature.

Additional information

Biodegradation in water

42-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical (Experimental study report, 2019). The study was performed at a temperature of 20°C. The test system included control, test chemical and reference chemical. Polyseed were used as a test inoculum for the study.The concentration of test and reference chemical (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test chemical and reference chemical. The % degradation of procedure control (reference chemical) was also calculated using BOD & ThOD and was determined to be 66.26%. Degradation of Sodium Benzoate exceeds 70.48% on 7 days & 73.49% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD42 value of test chemical was observed to be 0.75 mgO2/mg. ThOD was calculated as 2.64 mgO2/mg. Accordingly, the % degradation of the test chemical after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 28.4. Based on the results, the test item, under the test conditions, was considered to be inherently biodegradable in nature.