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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January 19 - February 22, 2012
1 (reliable without restriction)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guidelineopen allclose all
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
according to guideline
EPA OPPTS 835.3110 (Ready Biodegradability)
according to guideline
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-(3-chlorophenyl)-4-(3-chloropropyl)piperazinium chloride
EC Number:
EC Name:
1-(3-chlorophenyl)-4-(3-chloropropyl)piperazinium chloride
Cas Number:
Molecular formula:
1-(3-chlorophenyl)-4-(3-chloropropyl)piperazin-1-ium chloride
Test material form:
solid: particulate/powder
migrated information: powder
Details on test material:
Test item identification : 1-(3-chloroprophyl)-4-(3-chlorophenyl)-piperazine hydrochloride
Active ingredient content : 97.4 % w/w
Chemical formula : C13H18Cl2N2 * HCl
Chemical Class : pharma
C.A.S. number : 52605-52-4
Molecular weight : 309.65
Storage condition : The test item will be stored at room temperature in the dark conditions
Stability : Stable under the storage conditions.

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Details on study design:
The assay on test item has been run in parallel with a ready biodegradable reference item, Sodium Benzoate, an inoculum control, an abiotic control containing the test item and a sterilizing agent (HgCl2) and a toxicity control, containing test item, reference item and inoculum. BOD flasks of 500 mL capacity, equipped with pressure sensors fitted on the bottle mouth, have been used as test units.
Test solutions
Since the low water solubility of test item, the test item solutions (100 mg/L) were prepared by directly weighting into the test bottles, while the reference item and HgCl2 solutions were prepared by diluting an appropriate volume of an aqueous stock solutions of 10 g/L into test medium (10 mL of stock solutions of 10 g/L into 1000 mL of test medium). Activated sludge was added to each flask (except for abiotic control) and the flasks were made up to a volume of 250 mL or 150 mL, according to the scale setting of the manometric device, with the test medium
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
% degradation (O2 consumption)
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
under test conditions no biodegradation observed
The test item 1-(3-chloroprophyl)-4-(3-chlorophenyl)-piperazine hydrochloride has showed to be not ready biodegradable under the conditions applied in a Manometric Respirometry test, having not reached the pass level (60% biodegradation) but showing a biodegradation equal to 0% for 28 days of exposure.
Moreover, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the 25% of biodegradation exceeded in the toxicity control, already at the sixth day of test period.