C8-18 alkylampho(di)propionates

Short-term toxicity to fish

Description of key information

The acute toxicity of the substance to fish was investigated in 2 studies. In a 96 h acute toxicity study (classified as reliable without restrictions), carp (Cyprinus carpio) were exposed to the substance according to OECD 203 and GLP principles. The 96h-LC50 was 6.8 mg/L, corresponding with 2.6 mg solids/L, based on analytically confirmed nominal concentrations. In a 96 h acute toxicity study, of which the reliability could not be assigned, fish (Salmo salar) were exposed to the substance using a method equivalent/similar to OECD 203 but not under GLP principles. The 96h-LC50 was 2.3 mg/L, which corresponds to 0.9 mg solids/L, based on nominal concentrations (no analytical verification of test concentration).
The LC50 value obtained in the study classified as reliable without restrictions is taken as key value for CSA since it was considered the most reliable.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

6.8 mg/L

Additional information

The acute toxicity of the substance to fish was investigated in 2 studies:

Tobor-Kapłon (2013) - In a 96 h acute toxicity study, carp (Cyprinus carpio) were exposed to the substance according to OECD guideline Nr. 203 and GLP principles. Fish were exposed to the following nominal concentrations: 0 (control), 2.2, 4.6, 10, 22 and 46 mg/L, corresponding to 0.85, 1.8, 3.9, 8.5 and 18 mg solids/L.

The substance induced no mortality or other observed effects in carp at 4.6 mg/L, corresponding with 1.8 mg solids/L. The 96h-LC50 was 6.8 mg/L, corresponding with 2.6 mg solids/L based on analytically confirmed nominal concentrations (95% confidence interval between 4.6 and 10 mg/L, corresponding with 1.8 and 3.9 mg solids/L, respectively). The present toxicity study is classified as reliable without restrictions.

 

Grande (no date) – In a 96 h acute toxicity study, fish (Salmo salar) were exposed to the substance using a method equivalent/similar to OECD 203 but not under GLP principles. Fish were exposed to the following nominal concentrations: 0 (control), 1, 5, 10 and 50 mg/L, corresponding to 0.39, 1.95, 3.9 and 19.5 mg solids/L. The 96h-LC50 was 2.3 mg/L, which corresponds to 0.9 mg solids/L, based on nominal concentrations. The reliability of the study could not be assigned since the used test concentrations were not following the prescribed rules as at least 5 concentrations, arranged in a geometric series with a factor not exceeding 2.2, should have been selected. In addition, no analytical verification of the test concentrations was performed.

Selection of key study

The study conducted by Tobor-Kapłon (2013) was selected as the key study since it was considered the most reliable. The Grande (no date) study used test concentrations which did not followed the prescribed rules as at least 5 concentrations, arranged in a geometric series with a factor not exceeding 2.2, should have been selected. In addition, no analytical verification of the test concentrations was performed.