2-amino-2',5-dichlorobenzophenone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2',5-dichlorobenzophenone

EC Number:

220-985-2

EC Name:

2-amino-2',5-dichlorobenzophenone

Cas Number:

2958-36-3

Molecular formula:

C13H9Cl2NO

IUPAC Name:

4-chloro-2-(2-chlorobenzoyl)aniline

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

In order to get a saturated solution the test item was dispersed in test water in a concentration of 100 mg/L and stirred for 24 hours. The fine and unsolved
particles of the test item formulation were separated by filtration through a 0.22 µm membrane filter (bottle top filter with cellulose acetate membrane) to
avoid physical effects.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

c (Ca++)= 1.74 mmol/1
c (Mg++)= 0.43 mmol/1
CaC03 = 217 ppm

Test temperature:

18.7- 18.9°C

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass test aquaria of X mL volume filled with Y mL test medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 daphnia/40 mL of medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Potassium chloride: 5.75 mg/L
- Calcium chloride Dihydrate: 294.00 mg/L
- Magnesium sulphate Heptahydrate: 123.25 mg/L
- Sodium bicarbonate: 64.75 mg/L
- Conductivity: <5 µS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light/dark cycle was 15 to 9 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): swimming capability of Daphnia, 24 and 48 hours after beginning of exposure

TEST CONCENTRATIONS
- Test concentrations: 0.22mg/mL

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.21 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: all daphnids were mobile in the control during the 2 days of exposure.
- Any observations that might cause a difference between measured and nominal values: the test substance was soluble in medium, the test concentrations were present as a clear solution.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: EC50 (24 h) = 1.4 mg/L

Any other information on results incl. tables

Nominal conc. of test substance (mg/L):   0.21 mg/L	Number of immobilized Daphnia magna
	Exposure period  48 h	Exposure period  48 h	Exposure period  48 h	Exposure period  48 h	Total	Immobilisation (%)
Vessel	1	2	3	4
0 (control)	0	0	0	0	0	0
Test item	0	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this short-term toxicity study in daphnia magna an EC50 value of > 0.21 mg/L was found.