2-amino-2',5-dichlorobenzophenone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2',5-dichlorobenzophenone

EC Number:

220-985-2

EC Name:

2-amino-2',5-dichlorobenzophenone

Cas Number:

2958-36-3

Molecular formula:

C13H9Cl2NO

IUPAC Name:

4-chloro-2-(2-chlorobenzoyl)aniline

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: L17083
- Expiration date of the lot/batch: March, 2009


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient temperature, dry

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

The inoculum was derived from the effluent of the treatment plant Gemeinschaftsklärwerk Bitterfeld-W olfen receiving predominantly domestic
sewage. A fresh sample was allowed to settle for 1 hour and the decanted effluent was used for the test.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

3.7 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 20°C

SAMPLING
- Sampling frequency: Degradation was followed by oxygen evolution twice a week

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

3 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

6 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

10 d

% Degradation 5

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Kinetic of control substance:
51% after 3 days, 68% after 10 days, 76% after 13 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

In the present study no marked oxygen uptake by the test item was measured. The pass Ievel for ready biodegradation (60 per cent degradation) was not reached. For this reason in the test for ready biodegradability the test item seems to be not readily biodegradable.