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EC number: 811-213-0 | CAS number: 66711-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Remarks:
- Conducted according to guideline in effect at time of study conduct
- Qualifier:
- according to guideline
- Guideline:
- other: "Alga growth inhibition test, Daphnia sp. acute immobilisation test, and fish acute toxicity test" (Japanese notification, Yakushokuhatsu 0331 No.7, Heisei 23.03.29 Seikyoku No.5 , Kanpokihatsu No.110331009, March 31, 2011
- Deviations:
- no
- Remarks:
- Conducted according to guideline in effect at time of study conduct
- GLP compliance:
- yes
Test material
- Reference substance name:
- 66711-86-2
- Cas Number:
- 66711-86-2
- IUPAC Name:
- 66711-86-2
- Details on test material:
- - Purity: 99.7944%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 1) Preparation of standard solutions
About 45 mL dimethylsulfoxide was collected in a 50-mL volumetric bottle, and the bottle with dimethylsulfoxide was treated as a tare at weighing of the test substance. The test substance was collected into a 10-mL glass centrifuge tube from cylinder, then added into the 50-mL volumetric bottle by decantation and weighed subtracting the tare weight. After the weighing, this solution was filled up to 50 mL with dimethylsulfoxide. The concentration of the test substance was calculated based on the measured weight, and this solution was diluted sequentially with dimethylsulfoxide to prepare standard solutions of 4 concentrations within the range of approximately 10-1000 mg/L. Dimethylsulfoxide was used as the standard solution at 0 mg/L.
Analysis of Test Solutions
Samples of the test solutions at the beginning and the end of the renewal period, i.e. when it is freshly prepared and immediately prior to renewal or at the end of exposure are referred to as one set. All sets of test solutions were analysed. The test solution of each test group was collected and analysed by gas chromatography/mass spectrometry (GC/MS). Samples were prepared by mixing 0.1 mL of test solution and 10.0 mL of ultra pure water.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
I. Preparation of stock solution
For the preparation of the stock solution, 5.8 L of dechlorinated water (cooled in the refrigerator prior to use) was used as dilution water.
(I) 17.4 g of the test substance was collected into a 50-mL Erlenmeyer flask from the cylinder. (A 50-mL Erlenmeyer flask, a stopper and tape for sealing the stopper were treated as a tare. The test substance was weighed subtracting the tare weight.
(2) The test substance was added into the dilution water by decantation while stirring with a magnetic stirrer, and then stirred for an additional 30 minutes.
(3) The resulting stock solution was allowed to rest at room temperature for 30 minutes and the supernatant was collected by decantation (about 2 L).
( 4) The collected solution was warmed up to the test temperature, and sonicated for 2 minutes prior to its use for the preparation of the test solutions.
2. Preparation of test solutions
(I) For the preparation of the test solutions for control, dilution water was prepared by stirring 5L of cooled dechlorinated water, allowing it to rest at room temperature and sonicating in the same manner as the stock solution.
(2) For the preparation of the test solutions for Concentrations 1-5, the stock solution was mixed with dechlorinated water (approx 24°C) in appropriate amounts for each concentration.
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation: Less than a half year after hatching
- Feeding during test: no
ACCLIMATION PERIOD: 12 days
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 23.8 to 24.3 °C
- pH:
- 6.7 to 7.4
- Dissolved oxygen:
- control ranged from 7.5 to 9.5. mg/L in test solutions ranged from 6.5 to 8.6. mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 20, 39, 77, 150 and 300 mg/L
Measured concentrations: 0, 1.33, 2.60, 4.91, 9.56 and 20.7 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5-L glass vessel (cover: Teflon sheet), 5 L of test solution
- Renewal rate of test solution (frequency/flow rate): 24 hour renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Photoperiod: 16-hour light/8-hour dark
- Light intensity: 1000 lux or less
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Test fish were observed at 3, 24, 48, 72 and 96 hours after the start of exposure. Any fish found dead were removed promptly to prevent deterioration of water quality.
TEST CONCENTRATIONS
- Range finding study results: Range finding nominal test concentrations were: 3.0, 30, 300 and 3000 mg/L. Percent mortality at 96 hours was 0, 0, 100 and 100%, respectively.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence limits: 9.56-20.7 mg/L, Binomial method. The results were calculated from the time-weighted mean of the measured concentrations.
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test solutions appeared colourless, and suspended substances, floating substances and precipitated substances were not observed in the test solutions during exposure.
- Reported statistics and error estimates:
- The LC50 was calculated using the probit method, moving average method and binomial method (TOXDAT3_02). 95% Confidence limits were calculated.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
96-hour LC0: 9.56 mg/L
96-hour LC100: 20.7 mg/L
In the control group, the mortality at 96-hour exposure was 0%.
In mean measured test solutions 1.33, 2.60, 4.91 , 9.56 and 20.7 mg/L, mortality at 96 hours was 0, 0, 0, 0 and 100%, respectively. Abnormal symptom (abnormal swimming) was observed in one animal at 72 hours in test solution 20.7 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
96-h LC50 = 14.1 mgL - Executive summary:
An acute, semi-static 96-hour toxicity test (with renewal on a 24 period) was conducted at concentrations of 0, 20, 39, 77, 150 and 300 mg/L. The mean measured concentrations used in LC50 calculations were 0, 1.33, 2.60, 4.91, 9.56 and 20.7 mg/L. Because of the volatile nature of the test item, the test was performed in 5 L glass vessel covered with Teflon sheets containing 5 L of test item solution. Control and test solutions were conducted with one replicate with 10 Oryzias latipes in each. In all the test groups, the temperature of the test solutions was in the range of 23.8 to 24.3 °C, the D.O. values were 6.5 mg/L or more, and the pH values were 6.7 to 7.4, fulfilling the test conditions. No control animals died.
Based on the mean measured concentrations, the 96-hour LC50 for was 14.1 mg/L.
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