Registration Dossier
Registration Dossier
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Reaction mass of Potassium sodium 3-{[4-({3-(substitutedamino)-4-[(2-sulfonato-4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]phenyl}amino)-6-chloro-heteromonocyl-2-yl]amino}-2-substituted-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate (1:3:1) and Potassium sodium 3-[(4-{[3-(subsitutedamino)-4-{[4-(ethenylsulfonyl)-2-sulfonatophenyl]diazenyl}phenyl]amino}-6-chloro-heteromonocy-2-yl)amino]-2-substituted-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate (3:9:3)
EC number: - | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Determination of the acute fish toxicity on Rainbow trout with FAT 40863/A TE.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
This study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.
Methods…….
Following a preliminary range-finding test, fish were exposed, in
groups of seven, to an aqueous solution of the test item, at a single
concentration of 100 mg/L for a period of 96 hours at a temperature of
approximately 14 ºC under semi-static test conditions. The number of
mortalities and any sub-lethal effects of exposure in each test and
control vessel were determined 1, 3 and
6 hours after the start of exposure and then daily throughout the test
until termination after
96 hours.
Results…….
Analysis of the test preparations at 0, 24, 72 and 96 hours showed measured test concentrations to be near nominal and so it was considered justifiable to estimate the LC50values in terms of the nominal test concentrations.
Exposure of rainbow trout (Oncorhynchus mykiss)to the test item gave LC50values based on the nominal test concentrations of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.
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