Tetrasodium {5-[(4-chloro-6-{[4-(alkenylsulfonyl)phenyl]amino}-heteromonocycl-2-yl)amino]-4-(hydroxy-κO)-3-{[2-(hydroxy-κO)phenyl]diazenyl-κN}naphthalene-disulfonato(4-)}[3-(hydroxy-κO)-4-{[2-(hydroxy-κO)naphthalen-1-yl]diazenyl-κN}naphthalene-sulfonato(3-)]chromate(4-)

Short-term toxicity to fish

Description of key information

Determination of the acute toxicity of the test item (FAT 20341/A TE) to rainbow trout (Oncorhynchus mykiss).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

57 mg/L

Additional information

The study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). 

Following an initial experiment, fish were exposed, in groups of seven, to an aqueous solution of the test item over a range of concentrations of1.0,3.2,10,32, and 100 mg/L for a period of 96 hours at a temperature of approximately 14 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

 

Analysis of the test preparations at 0 and 72 hours (fresh media) and at 24 and 96 hours (old media) showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only.

 

Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave the following results based on the nominal test concentrations:

 

Time Point (Hours)	LC50 (mg/L)	95% Confidence Limits (mg/L)			No Observed Effect Concentration (NOEC) (mg/L)	Lowest Observed Effect Concentration (LOEC) (mg/L)
96	57	32	-	100	32	100