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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from authoritative database and secondary source

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
IUCLID Dataset
Author:
European Chemicals Bureau (ECB)
Year:
2000
Bibliographic source:
IUCLID Dataset, European Chemicals Bureau (ECB), 2000
Reference Type:
other: Authoritative database
Title:
HSDB for the Test Chemical
Author:
U S National Library of Medicine
Year:
2007
Bibliographic source:
Hazardous Substances Data Bank [HSDB], last updated 2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To assess the eye irritation potential of the test chemical in rabbits
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium hydrogen glutamate
EC Number:
205-538-1
EC Name:
Sodium hydrogen glutamate
Cas Number:
142-47-2
Molecular formula:
C5H9NO4.Na
IUPAC Name:
Monosodium glutamate
Test material form:
solid
Details on test material:
- Name: Monosodium glutamate
- Name of test material (as cited in study report): L-glutamic acid sodium salt
- Molecular formula: C5H8NNaO4
- Molecular weight: 169.1112 g/mol
- Smiles notation: [C@H](CCC(=O)O)(C(=O)[O-])N.[Na+]
- InChl : 1S/C5H9NO4.Na/c6-3(5(9)10)1-2-4(7)8;/h3H,1-2,6H2,(H,7,8)(H,9,10);/q;+1/p-1/t3-;/m0./s1
- Substance type: Organic
- Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
no data available

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
Approximately 70 mg of the test substance, the weight occupying a volume of 0.1 ml
Duration of treatment / exposure:
single exposure
Observation period (in vivo):
2 days
Duration of post- treatment incubation (in vitro):
no data available
Number of animals or in vitro replicates:
no data available
Details on study design:
no data available

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
mean
Time point:
other: 1 hours
Score:
2
Reversibility:
not specified
Remarks on result:
probability of weak irritation
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 1 hour
Score:
2
Reversibility:
not specified
Remarks on result:
probability of weak irritation
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
mean
Time point:
24 h
Score:
1
Reversibility:
not specified
Remarks on result:
probability of weak irritation
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
48 h
Score:
0
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No corneal damage or iridal inflammation was observed. Considering on the one hand the neutral pH of MSG (in aqueous solution) and, on the other hand, the fact that MSG is marketed under a crystalline form, the transient, benign conjunctival irritation observed is likely attributable to mechanical effects.

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
No corneal damage or iridal inflammation was observed. One hour after instillation, conjunctiva redness and conjunctiva chemosis scores were 2.After one day the conjunctival redness score decreased to grade 1 and the eyes were normal after 2 days. The transient, benign irritation was attributed to the mechanical effects than the chemical(since it has neutral range pH).
Hence, the test chemical can be considered to not irritating to rabbit eyes.
Executive summary:

The ocular irritation potential of the test chemical was assessed in rabbits. Approximately 70 mg of the test substance, the weight occupying a volume of 0.1 ml was instilled in the eyes of rabbits and observed for signs of irritation till 2 days.

 

No corneal damage or iridal inflammation was observed.One hour after instillation, conjunctiva redness and conjunctiva chemosis scores were 2.After one day the conjunctival redness score decreased to grade 1 and the eyes were normal after 2 days. The transient, benign irritation was attributed to the mechanical effects than the chemical(since it has neutral range pH).

Hence, the test chemical can be considered to not irritating to rabbit eyes.