Disodium succinate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Toxicity of test material to Bacteria Klebsiella pneumoniae H12 was evaluated in the study for the duration of 96 hr.The toxicity is evaluated based on Effect concentration observed.

GLP compliance:

no

Analytical monitoring:

not specified

Details on sampling:

405.13,810.26,1620.52,3241.04,6482.08, 12964.16mg/l

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

other: Klebsiella pneumoniae H12

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

4 d

Hardness:

No data available

Test temperature:

28⁰ C

pH:

7.0

Salinity:

No data available

Nominal and measured concentrations:

405.13,810.26,1620.52,3241.04,6482.08, 12964.16mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open / closed No data available
- Material, size, headspace, fill volume: No data available
- Aeration: Yes, with a totary shaker (250 rpm with a 50-mm stroke)
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): No data available
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: Yes, pH was adjusted to 7.0
- Photoperiod: No data available
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Polysaccharide productivity

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study No data available
- Test concentrations: 2.5, 5.0, 10.0, 20.0, 40.0, 80.0 mM (4051.3, 8102.6, 16205.2, 32410.4, 64820.8, 129641.6 mg/L)
- Results used to determine the conditions for the definitive study: No data available

Duration:

4 d

Dose descriptor:

other: EC

Effect conc.:

3 241.04 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Decrease in the polysaccharide productivity

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Conclusions:

The EC for the test material was found to be 20mM (3241.04 mg/L).

Executive summary:

Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing  30 ml  of  YPE  medium. Production of the polysaccharide was measured by the dry weight after ethanol  precipitation   of  the  culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).

Description of key information

Toxicity to microorganism:

Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing  30 ml  of  YPE  medium. Production of the polysaccharide was measured by the dry weight after ethanol  precipitation   of  the  culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).

Additional information

Toxicity to microorganism:

Effect of test material was evaluated on microorganisms based on the data of target chemical from peer reviewed journal.

Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing  30 ml  of  YPE  medium. Production of the polysaccharide was measured by the dry weight after ethanol  precipitation   of  the  culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).

In another study , Klebsiella pneumonieH12 is a bacteria and polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using sodium succinate. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and title comp was studied. In the flocculation assay -in a test tube, 9 ml of a kaolin clay suspension (5.5 g /l) was mixed with 1 ml of polysaccharide solution, 100 µl of or- ganic acid solution, and 100 µl CaCl2.2H2O solution in this sequence with a Vortex mixer for 5 sec. After 5 min, OD_550c of the supernatant was measured. A control experiment was done in the absence of the polysaccharide and the OD_550cwas measured. Flocculation of bacterial cells by polysaccharide was inhibited at a concentration of 0.3mM (48.61 mg/l) by the test compound .The EC for the test material Sodium succinate is thus found to be 0.3mM (48.61 mg/l).