4-Nonylphenol, ethoxylated

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-CHECK authoritative database

Qualifier:

according to guideline

Guideline:

other: Biodegradation test of a chemical substance using a microorganism etc.

Principles of method if other than guideline:

Biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance 4-Nonylphenol, ethoxylated.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material :4-Nonylphenol, ethoxylated
- Molecular formula : C17H28O2
- Molecular weight :264.406 g/mol
- Smiles notation : c1(ccc(OCCO)cc1)CCCCCCCCC
- Substance type: Organic
- Physical state: Liquid
- Other: addition molar number of ethylene oxide ca. 10
- Water solubility: <= 30%

Oxygen conditions:

not specified

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

- Concentration of sludge: 30 ppm (30 mg/L)

Duration of test (contact time):

3 wk

Initial conc.:

30 mg/L

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

, BOD, TOC removal, test material analysis, UV-Vis

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

3 wk

Remarks on result:

other: Other details not known

Key result

Parameter:

% degradation (TOC removal)

Value:

14.3

Sampling time:

3 wk

Remarks on result:

other: Other details not known

Key result

Parameter:

% degradation (test mat. analysis)

Remarks:

and UV-Vis

Value:

0

Sampling time:

3 wk

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 14.3% degradation by TOC removal parameter in 21 days.

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The percentage degradation of test substance was determined to be 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 14.3% degradation by TOC removal parameter in 21 days. Thus, based on percentage degradation, 4-Nonylphenol, ethoxylated was considered to be not readily biodegradable in water.

Executive summary:

Biodegradation study was conducted for 21 days for evaluating the test substance 4-Nonylphenol, ethoxylated (CAS no. 26027 -38 -3). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 14.3% degradation by TOC removal parameter in 21 days. Thus, based on percentage degradation, 4-Nonylphenol, ethoxylated was considered to be not readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance 4-Nonylphenol, ethoxylated (CAS no. 26027 -38 -3) (J-CHECK, 2017). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 14.3% degradation by TOC removal parameter in 21 days. Thus, based on percentage degradation, 4-Nonylphenol, ethoxylated was considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Various experimental and predicted data for the target compound 4-Nonylphenol, ethoxylated (CAS No. 26027-38-3) and supporting studies for its read across were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from authoritative database (J-CHECK, 2017), biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance 4-Nonylphenol, ethoxylated (CAS no. 26027 -38 -3). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 14.3% degradation by TOC removal parameter in 21 days. Thus, based on percentage degradation, 4-Nonylphenol, ethoxylated was considered to be not readily biodegradable in nature.

 

Another biodegradation study was conducted for 21 days for evaluating the percentage biodegradability of test substance 4-Nonylphenol, ethoxylated (CAS no. 26027 -38 -3) (J-CHECK, 2017). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% degradation by BOD, O2 consumption, test mat. analysis and UV-Vis and 10.3% degradation by TOC removal parameter in 21 days. Thus, based on percentage degradation, 4 -Nonylphenol, ethoxylated was considered to be not readily biodegradable in nature.

 

In a prediction using the Estimation Programs Interface Suite (EPI suite, 2017), the biodegradation potential of the test compound  4-Nonylphenol, ethoxylated (CAS no. 26027 -38 -3) in the presence of mixed populations of environmental microorganisms was estimated. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 4-Nonylphenol, ethoxylated is expected to be not readily biodegradable.

 

For the read across chemical Polyoxyethylene( 15)tallow amine (CAS no. 61791-26-2) from peer reviewed journal (C. G. van Ginkel et. al; 1993), the biodegradation study was carried out using prolonged closed bottle test for testing percent biodegradation of read across chemical Polyoxyethylene( 15)tallow amine according to OECD guideline 301D at temperature 20oC. Secondary activated sludge was used as inoculums. The percent biodegradation obtained for chemical Amines, ethoxylated(1 - 4.5 moles ethoxylated) of CAS no. 61791-26-2 was 28 % in 28 days and 67 % in 214 days According to these percentage value and duration it is concluded that the chemical Amines, ethoxylated (1 - 4.5 moles ethoxylated) of CAS no. 61791-26-2 is not readily biodegradable..

 

On the basis of above results for target chemical 4 -Nonylphenol, ethoxylated (from authoritative database J-CHECK and EPI suite, 2017) and for its read across substance (from peer reviewed journal), it can be concluded that the test substance 4 -Nonylphenol, ethoxylated can be expected to be not readily biodegradable in nature.