Benzyl 4-hydroxybenzoate

Short-term toxicity to fish

Description of key information

Acute toxicity test was performed on fish for 96 hrs at 5 different concentrations according to the OECD guideline 203.10 fishes of 10 days old were used in 100 ml beaker. Half of the paraben solution was replaced every 24 h. Specific environmental condition was maintained and LC 50 value was determined.After experiment it was observed that the LC 50 value for benzylparaben(94-18-8) to fish was found to be 0.73mg/l concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.73 mg/L

Additional information

Various studies were reviewed along with the prediction data to evaluate the effect of the test compound on toxicity to fish. These are summarized as follows:

 

Acute toxicity test was performed on fish for 96 hrs at 5 different concentrations according to the OECD guideline 203.10 fishes of 10 days old were used in 100 ml beaker. Half of the paraben solution was replaced every 24 h. Specific environmental condition was maintained and LC 50 value was determined.After experiment it was observed that the LC 50 value for benzylparaben(94-18-8) to fish was found to be 0.73mg/l concentrations.

 

From peer reviewed journal the short-term toxicity to Pimephales promelas was performed for 48 hrs at five nominal concentrations in a temperature-controlled incubator at 24°C with a 16:8 light: dark cycle.500 ml beaker was taken having 250 ml of test chemical. Test solution was prepared by dissolved in high-performance liquid chromatography grade acetone and delivered into RHW at 0.1% acetone. No aeration was used and LD 50 value was determined at 24 and 48 hrs. On the basis of above experiment result the LD 50 value for Pimephales promelas for benzylparaben (94-18-8) was found to be 3.3 mg/L concentration.

 

The short term toxicity to fish test was performed on Oryzias latipes according to the OECD guideline 203. Fish was bred, acclimatized and exposed to atleast six different concentrations in a 100 ml beaker. Half of the test solution was replaced every 24 hrs. Mortality was measured and LC50 value was determined using probit conversion analysis.

According to the observations the LC 50 value for benzylparaben (94-18-8) to Oryzias latipes was found to be 0.72 mg/l concentration.

From above mentioned supporting studies the target chemicalbenzylparaben(CAS No 94-18-8), the majority values are indicative that the chemical is likely to cause aquatic fish toxicity.