(E)-3,7-dimethylocta-2,6-dienal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed according to EU guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 79/831 EWG, C2 annex V

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Citral

EC Number:

226-394-6

EC Name:

Citral

Cas Number:

5392-40-5

IUPAC Name:

3,7-dimethylocta-2,6-dienal

Details on test material:

- Name of test material (as cited in study report): Citral N
Chemical name : 3,7-Dimethyl-2,6-octadien-l-al
- Analytical purity: 93.5% Cis / Trans mix, 1.2% Prenol, 0.2% MBA, 1-2% Iso - Citrate
- Lot/batch No.: B 2460 11.04.88
Solubility in water: poor solubility

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A concentrated stock solution of the test substance in test medium was prepared by adding Tween 80 as a solvent deflocculant (nominal 50 mg/l test substance and 100 mg/l solvent) and addition stirring for 15 min at room temperature.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978
- Age at study initiation (mean and range, SD): < 24 h
ACCLIMATION
- Type and amount of food: bakers yeast (after water change), green algae
- Feeding frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l

Test temperature:

20 ± 1 °C

pH:

7.29 - 8.4

Dissolved oxygen:

5.28 - 8.69 mg/l

Nominal and measured concentrations:

Nominal test concentrations:
0.3906, 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, control, solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glasses
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Ca/mg ratio: 4:1
- Conductivity: 550-650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

3.13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Results after 24 hours (in mg/l): EC50: 10.71 (confidence intervals: 7.45 - 13.97) EC0: 6.25 EC100: 25.0