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Toxicity to soil microorganisms

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Endpoint:
toxicity to soil microorganisms
Remarks:
Collembola
Type of information:
experimental study
Remarks:
Field study
Adequacy of study:
key study
Study period:
1985-1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
no guideline available
Principles of method if other than guideline:
Field study
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
water
Details on preparation and application of test substrate:
The substance was stirred into 600 l water/ha and applied to the trial area immediately after the urea application.
This was followed by incorporation into the soil.
Test organisms (inoculum):
soil
Total exposure duration:
72 mo
Nominal and measured concentrations:
Nominal 2, 4, 8 kg CPMP/ha
Key result
Duration:
132 wk
Dose descriptor:
NOEC
Effect conc.:
> 4 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
nitrate formation rate
Remarks:
number of microrganisms
Validity criteria fulfilled:
yes
Conclusions:
The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.
Executive summary:

The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1985-1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
no guideline available
Principles of method if other than guideline:
Field study
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
water
Details on preparation and application of test substrate:
The substance was stirred into 600 l water/ha and applied to the trial area immediately after the urea application.
This was followed by incorporation into the soil.
Test organisms (inoculum):
soil
Total exposure duration:
72 mo
Nominal and measured concentrations:
nominal 2,4,8 kg/ha
Key result
Duration:
312 wk
Dose descriptor:
NOEC
Effect conc.:
> 4 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
nitrate formation rate
Remarks:
number of microorganisms
Validity criteria fulfilled:
yes
Conclusions:
The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.
Executive summary:

The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.

Endpoint:
toxicity to soil microorganisms
Remarks:
Collembola
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1989-1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
CMP (1-carbamoyl-3-methylpyrazole) undergoes relatively rapid hydrolysis to 3-methylpyrazole (MP) in both water and soil.
In soil, the half-life for the conversion of CMP to 3-MP was 1-2.5 days.
The half-life for hydrolysis in aqueous solution was 35.0 - 85.5 days.
Therefore the study results for CMP for toxicity to terestrial organisms can be read-across to 3-methylpyrazole.
For detailed information of degradation and hydrolysis please see attached study report.
Reason / purpose for cross-reference:
read-across source
Qualifier:
no guideline available
Principles of method if other than guideline:
Field study
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
water
Details on preparation and application of test substrate:
The substance was stirred into 600 l water/ha and applied to the trial area immediately after the urea application.
This was followed by incorporation into the soil.
Test organisms (inoculum):
soil
Total exposure duration:
72 mo
Nominal and measured concentrations:
nominal 2, 4, 8 kg CMP/ha
Key result
Duration:
132 wk
Dose descriptor:
NOEC
Effect conc.:
> 2.63 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
other: 3-methylpyrazole recalculated from CMP
Basis for effect:
nitrate formation rate
Remarks:
number of microorganisms
Validity criteria fulfilled:
yes
Conclusions:
The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.
Executive summary:

The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.

Endpoint:
toxicity to soil microorganisms
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1984-1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
CMP (1-carbamoyl-3-methylpyrazole) undergoes relatively rapid hydrolysis to 3-methylpyrazole (MP) in both water and soil.
In soil, the half-life for the conversion of CMP to 3-MP was 1-2.5 days.
The half-life for hydrolysis in aqueous solution was 35.0 - 85.5 days.
Therefore the study results for CMP for toxicity to terestrial organisms can be read-across to 3-methylpyrazole.
For detailed information of degradation and hydrolysis please see attached study report.
Reason / purpose for cross-reference:
read-across source
Qualifier:
no guideline available
Principles of method if other than guideline:
Guideline not availabe, field study
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
water
Details on preparation and application of test substrate:
The substance was stirred into 600 l water/ha and applied to the trial area immediately after the urea application.
This was followed by incorporation into the soil.
Test organisms (inoculum):
soil
Total exposure duration:
72 mo
Key result
Duration:
312 wk
Dose descriptor:
NOEC
Effect conc.:
> 2.63 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
other: 3-methylpyrazole recalculated from CMP
Basis for effect:
nitrate formation rate
Remarks:
additional count of microorganisms
Validity criteria fulfilled:
yes
Conclusions:
The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.
Executive summary:

The investigations to determine the influence of a CMP continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.

Description of key information

The investigations to determine the influence of a CMP which is rapidly decomposed to 3-methylpyrazole, continuous treatment on the abundance of selected soil organisms and the nitrification activity of the soils depending on the N fertiliser type and the application period autumn/spring at different sites yielded the following result:
CMP has no influence on the population density of microorganisms and small arthropods in the soil.

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
2.63 mg/kg soil dw

Additional information