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Registration Dossier
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EC number: 202-255-5 | CAS number: 93-53-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- STUDY OF ARTIFICIAL FLAVOURING SUBSTANCES FOR MUTAGENICITY IN THE SALMONELLA/MICROSOME, BASC AND MICRONUCLEUS TESTS.
- Author:
- D. WILD, M.-T. KING, E. GOCKE and K. ECKHARDT
- Year:
- 1 983
- Bibliographic source:
- Fd Chem. Toxic'. Vol. 21, No. 6, pp. 707-719, 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below principle
- Principles of method if other than guideline:
- Toxicity of 2 Phenylpropionaldehyde was study using Salmonella/mammalian microsome test (Ames test).
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- Hydratropaldehyde
- EC Number:
- 202-255-5
- EC Name:
- Hydratropaldehyde
- Cas Number:
- 93-53-8
- Molecular formula:
- C9H10O
- IUPAC Name:
- hydratropaldehyde
- Reference substance name:
- 2 Phenylpropionaldehyde
- IUPAC Name:
- 2 Phenylpropionaldehyde
- Test material form:
- other: Liquid
- Details on test material:
- Details on test material
- Name of test material (as cited in study report): 2 Phenylpropionaldehyde (synonym-Hydratropic aldehyde)
- Molecular formula (if other than submission substance): C9-H10-O
- Molecular weight (if other than submission substance): 134.177 g/mol
- Substance type: Organic
- Physical state: Liquid
- Purity: No data available
- Impurities (identity and concentrations): No data available
Constituent 1
Constituent 2
Method
- Target gene:
- No data available
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 1535, TA 100, TA 1537,TA1538 and TA98
- Details on mammalian cell type (if applicable):
- No data available
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor-pretreated rats
- Test concentrations with justification for top dose:
- up to 3.6 mg/plate(Five doses were taken)
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- Remarks:
- No other data available
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: plate incorporation method using Vogel Bonnet Medium
DURATION
- Preincubation period: No data available
- Exposure duration: 48 hrs
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS:
No data available
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other:
OTHER: - Evaluation criteria:
- numbers of revertants/plate were calculated
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA 1535, TA 100, TA 1537,TA1538 and TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: Negative (with and without)
After experiment, end point for genetic toxicity for 2 Phenylpropionaldehyde(93-53-8) was determined to be negative with and without activation. - Executive summary:
2 Phenylpropionaldehyde artificial flavouring substance in food products was studied for mutagenic properties by the use of the Salmonella/mammalian microsome test (Ames test).
The test was performed by plate incorporation method at 5 different dosesupto 3600 µg/plate and the plates were incubated for 48hrs.
2 Phenylpropionaldehyde is non mutagenic when Salmonella/microsome AMES test is performed.
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