Registration Dossier
Registration Dossier
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EC number: 201-377-6 | CAS number: 81-81-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparative Acute Toxicity of the First 50 Multicentre Evaluation of In Vitro Cytotoxicity Chemicals to Aquatic Non-Vertebrates
- Author:
- Calleja MC, Persoone G, Geladi P
- Year:
- 1 994
- Bibliographic source:
- Archives of Environmental Contamination and Toxicology, 26, 69-78
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Guideline:
- other: manufacturer's SOP
- Deviations:
- no
- Remarks:
- - Performed to standard assay procedures (Microbics 1989.)
- GLP compliance:
- no
Test material
- Reference substance name:
- Warfarin
- EC Number:
- 201-377-6
- EC Name:
- Warfarin
- Cas Number:
- 81-81-2
- Molecular formula:
- C19H16O4
- IUPAC Name:
- 4-hydroxy-3-(3-oxo-1-phenylbutyl)-2H-chromen-2-one
- Details on test material:
- - Name of test material (as cited in study report): Warfarin.
- Analytical purity: >97 %.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Photobacterium phosphoreum
- Details on inoculum:
- Not specifed.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 5 min
- Remarks on exposure duration:
- 5-15 minutes
- Post exposure observation period:
- None.
Test conditions
- Hardness:
- Not specified.
- Test temperature:
- Not specified.
- pH:
- Not specified.
- Dissolved oxygen:
- Not specified.
- Salinity:
- Not specified.
- Nominal and measured concentrations:
- Not specified.
- Details on test conditions:
- Not specified.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 5 min
- Dose descriptor:
- EC50
- Effect conc.:
- 219 µmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 95 % CI not given
- Details on results:
- Not specified.
- Reported statistics and error estimates:
- EC50 obtained by linear regression package provided by the Microbics Corporation (manufacturer of the Microtox product).
Any other information on results incl. tables
The 5 and 15 minute EC50s of the Microtox test were obtained by the linear regression package provided by the Microbics Corportation.
Applicant's summary and conclusion
- Conclusions:
- The reported EC50 was 219 µmol/L on Photobacterium phosphoreum using the Microtox(TM) test.
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