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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

skin irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
no guideline available
Principles of method if other than guideline:
The test consists of a topical exposure of the neat test item to a human reconstructed epidermis model followed by a cell viability test. Cell viability is measured by dehydrogenase conversion of MTT [3,4,5.dimethyl thiazole 2-yl) 2,5-diphenyl-tetrazoliumbromide], present in cell mnithocondria, into a blue formazan salt that is quantitatively measured after extraction from tissues. The percent reduction of cell viability in comparison of untreated negative controls is used to predict skin irritation potential.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(4Z)-hept-4-en-2-yl salicylate
EC Number:
Cas Number:
Molecular formula:
(4Z)-hept-4-en-2-yl salicylate

Test animals

other: tissue of skin model EpiSkin

Test system

Type of coverage:
unchanged (no vehicle)
Duration of treatment / exposure:
15 min

Results and discussion

Any other information on results incl. tables

This in vitro study was performed to assess the potential of Gr-86 -3792 by means of the Human Skin Model Test.

Three tissues of the human skin model EpiSkin were treated with either the test item, the negative control or the positive control for 15 min.

15 microL of the test item were applied to each tissue spread to match the tissue size.

15 microL of either the negative control (deionised water) or the positive control (5% sodium Lauryl sulfate) were applied to each tissue.

After treatment with the negative control induced a sufficient decrease in the relative absorbance as compared to the negative control for the 15 min treatment interval thus ensuring the validity of the test system.

After treatment with the test item GR-86 -3792 the relative absorbance valuees were decreased to 88.2%. This value is well above above the threshold for irritancy of <= 50%.Therefore, the test item is not considered to possess an irritant potential.

In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item GR-86 -3792 is non irritant to skin.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Migrated information Criteria used for interpretation of results: EU