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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal
Justification for type of information:
Data is from peer reviewed journal
Qualifier:
according to guideline
Guideline:
other: As mention below
Principles of method if other than guideline:
This study was designed to access the effect of the substance 2,4,6-trichloroaniline on the luminescent bacteria Photobacterium phosphoreum carried out for 30 minutes.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): 2,4,6-trichloroaniline
- Molecular formula (if other than submission substance): C6-H4-Cl3-N
- Molecular weight (if other than submission substance): 196.464
- Smiles notation (if other than submission substance): c1(c(cc(Cl)cc1Cl)Cl)N
- InChl (if other than submission substance): 1S/C6H4Cl3N/c7-3-1-4(8)6(10)5(9)2-3/h1-2H,10H2
- Substance type: organic
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Photobacterium phosphoreum
Test type:
not specified
Water media type:
freshwater
Total exposure duration:
30 min
Remarks on exposure duration:
5, 15 and 30 minutes
Reference substance (positive control):
not specified
Key result
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
4.61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of luminescence
Remarks on result:
other: Other details not known
Validity criteria fulfilled:
not specified
Conclusions:
Based on the inhibition of luminescence of Photobacterium phosphoreum, the EC50 was observed at 4.61 mg/l.
Executive summary:

This study was designed to access the effect of the substance 2,4,6-trichloroaniline on the luminescent bacteria Photobacterium phosphoreumcarried out for 30 minutes. Before testing chemical was analyzed by HPLC. Based on the inhibition of luminescence of Photobacterium phosphoreum, the EC50 was observed at 4.61 mg/l. Based on the EC50, chemical can be consider as toxic.

Description of key information

Study was designed to access the effect of the substance 2,4,6-trichloroaniline on the luminescent bacteria Photobacterium phosphoreum carried out for 30 minutes. Before testing chemical was analyzed by HPLC. Based on the inhibition of luminescence of Photobacterium phosphoreum, the EC50 was observed at 4.61 mg/l. Based on the EC50, chemical can be consider as toxic.

Key value for chemical safety assessment

EC50 for microorganisms:
4.61 mg/L

Additional information

Based on the experimental data from various database for the target chemical study have been reviewed to determine the mode of action of2,4,6-trichloroaniline (CAS no. 634-93-5)on the growth and other activity of microorganisms. The studies are as mentioned below:

 

In the first key study from peer reviewed journal 2007, study was designed to access the effect of the substance 2,4,6-trichloroaniline on the luminescent bacteria Photobacterium phosphoreum carried out for 30 minutes. Before testing chemical was analyzed by HPLC. Based on the inhibition of luminescence of Photobacterium phosphoreum, the EC50 was observed at 4.61 mg/l. Based on the EC50, chemical can be consider as toxic.

 

First key study was supported by the second study from secondary sources 1987, 30 minutes study was conducted in which toxicity were measured on the basis of inhibition of luminescence of Pseudomonas putida. Based on the inhibition of cell multiplication after 30 minutes exposures, the EC10 of the test material 2, 4, 6-trichloroaniline is observed to be > 21 mg/L in Pseudomonas putida.

 

Similar study from same sources 30 minutes study was conducted in which toxicity were measured on the basis of inhibition of luminescence of Photobacterium phosphoreum. Toxicity effect of the test material 2, 4, 6-trichloroaniline was studied in Photobacterium phosphoreum. The toxicity effect were observed based on inhibition of luminescence during 30 minutes period. The EC50 is determine to be 4.60 mg/L.

 

Based on the above data, it was observed that the toxicity value ranges from 4.60 to > 21 mg/l.