2-Acetone, polymer with phenol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: the study was conducted according to O.E.C.D. Testing Guideline No. 301 F with GLP compliance including test substance characterization.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-isopropylidenediphenol

EC Number:

201-245-8

EC Name:

4,4'-isopropylidenediphenol

Cas Number:

80-05-7

Molecular formula:

C15H16O2

IUPAC Name:

2,2-bis(4-hydroxyphenyl)propane

Constituent 2

Reference substance name:

4,4’-isopropylidenediphenol

IUPAC Name:

4,4’-isopropylidenediphenol

Constituent 3

Reference substance name:

Phenol, 4,4’-(1-methylethylidene)bis-

IUPAC Name:

Phenol, 4,4’-(1-methylethylidene)bis-

Constituent 4

Reference substance name:

Bisphenol A

IUPAC Name:

Bisphenol A

Details on test material:

As per IUCLID5 Sections 1.1. -1.4. for Bisphenol A.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
As per IUCLID5 Sections 1.1. -1.4. for Bisphenol A.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge mixed liquor was obtained from the City of Midland Wastewater Treatment Pland (Midland, Michigan)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

7 - 25 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

and CO2 production.

Details on study design:

Duplicate reaction flasks contained 30 mg/L activated sludge in defined mineral salts medium. The concentrations of Bisphenol a were 7 and 25 mg/L. The reaction flasks were incubated in darkness in a circulated air incubator with continous stirring at approximately 200 rom at 22.5 +/- 0.2 degrees C. Sodium benzoate at 100 mg/L was the positive control. Duplicate inoculum blanks (mineral salts medium only) and toxicity controls were also established.

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/L

Results and discussion

Preliminary study:

An initial study was conducted at a temperature of 27.1 +/- 0.6 degrees C. This elevated incubation temperature had little or no impact upon biodegradation kinetics.

% Degradation

Parameter:

% degradation (O2 consumption)

Remarks:

at 25 mg/L BPA.

Value:

ca. 81

Sampling time:

28 d

Remarks on result:

other: Levels of biodegradation were similar for 7 mg/L Bisphenol A.

Details on results:

After an initial lag period of 4.7 to 6.1 days, a 60% biodegradation of Bisphenol A was achieved within 5 to 7 days of contact time. The biodegradation of 7 mg/L Bisphenol A ranged from 74.7 to 93.5% after 28 days. The biodegradation of 25 mg/L of Bisphenol A ranged from 75.9 to 93.5 % after 28 days of contact time.

BOD5 / COD results

Results with reference substance:

The biodegradation of sodium benzoate was 75.8 to 99.5% complete at 28 days and required 2.1 to 2.5 days to react 60% biodegradation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Bisphenol A, a structural analog of 2-Acetone polymer with phenol (BPA-Tars) was "readily biodegradable" reaching a level of 60% biodegradation within a window of 5 to 7 days contact time. It is anticipated that BPA-Tars would achieve a similar level of biodegradation under the same experimental conditions.

Executive summary:

A structural analog of BPA-Tars, Bisphenol A was assessed for ready biodegradability by an O.E.C.D. 301 F Testing Guideline study conducted according to the GLP regulations. Bisphenol A, was "readily biodegradable" reaching a level of 60% biodegradation within a window of 5 to 7 days contact time. It is anticipated that BPA-Tars would achieve a similar level of biodegradation under the same experimental conditions.