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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
No data
Vehicle:
not specified
Details on test solutions:
M4 medium
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: RCC Ltd.
- Age at study initiation (mean and range, SD): Young daphnids, aged less than ‘24’ hours old
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
220 mg/L
Test temperature:
20.7- 20.8 °C
pH:
7.69 - 7.88
Dissolved oxygen:
7.63 – 7.78 mg/L
Salinity:
Not available
Conductivity:
Not available
Nominal and measured concentrations:
0.30, 0.60, 1.20 2.40, 4.80, 9.60 and 19.20 mg/L
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10 daphnids
Reference substance (positive control):
yes
Remarks:
Potassium Dichromate Pure
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
1.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
Based on the test results, the EC50 of Potassium Dichromate Pure in Daphnia magna observed for a period of ‘24’ hour was calculated as 1.72 mg/L with upper confidence limits of 2.12 mg/L and lower confident limits of 1.40 mg/L and regression equation Y = 4.0957 + 3.8604 log (x). At ‘48’ hour, the EC50 was calculated as 1.33 mg/L with upper confidence limits of 1.63 mg/L and lower confidence limits of 1.08 mg/L and regression equation Y = 4.5239 + 3.8769 log (x).
Reported statistics and error estimates:
None

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24 hour, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilisation at the end of 24 hour was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48 hour, 3/40, 7/40 and 14/40 immobility was recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus the percent cumulative immobilisation at the end of 48 hour was recorded to be 0% in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

Based on the test results, the EC50 of  FAT 20290/C TE  in Daphnia magna observed for a period of 24 hour was calculated as 24.07 mg/L with upper confidence limits of 43.27 mg/L and lower confidence limits of 18.27 mg/L and regression equation Y = 0.8956 +2.9711 log (x).

The EC50 of FAT 20290/C TE  in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64  mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 24 hours was calculated as 4.80 mg/L and 9.60 mg/L respectively.

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L  and 1.20 mg/L respectively.

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L.
Executive summary:

Acute Immobilization Toxicity Study in Daphnia magna was conducted in accordance with OECD guideline 202 with FAT 20290/C TE. Based on the results of the range finding experiment, main experiment was conducted with a range of concentrations viz., 0.30, 0.60, 1.20, 2.40, 4.80, 9.60 and 19.20 mg/L.

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24-hour observation period, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilization at the end of 24-hour observation period was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48-hour observation period, 3/40, 7/40 and 14/40 immobile animals were recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus, the percent cumulative immobilization at the end of 48 hour was recorded to be 0 % in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64 mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L and 1.20 mg/L, respectively.

Description of key information

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hours was calculated as 4.40 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
4.4 mg/L

Additional information

Acute Immobilization Toxicity Study in Daphnia magna was conducted in accordance with OECD guideline 202 with FAT 20290/C TE. Based on the results of the range finding experiment, main experiment was conducted with a range of concentrations viz., 0.30, 0.60, 1.20, 2.40, 4.80, 9.60 and 19.20 mg/L.

No immobilization was observed in control and daphnids exposed to 0.30 and 0.60 mg/L test concentration throughout the experimental period. At the end of 24-hour observation period, no immobility was observed in daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 7/40 and 14/40 immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. Thus, the percent immobilization at the end of 24-hour observation period was recorded to be 0 % in control and daphnids exposed to 0.30, 0.60, 1.20, 2.40 and 4.80 mg/L concentrations whereas, 17.5 % and 35 % was observed in daphnids exposed to 9.60 and 19.20 mg/L concentrations respectively. At the end of 48-hour observation period, 3/40, 7/40 and 14/40 immobile animals were recorded in the daphnids exposed to 1.20, 2.40 and 4.80 mg/L concentration whereas, 39/40, 40/40 cumulative immobility was observed in daphnids exposed to 9.60 and 19.20 mg/L concentration. Thus, the percent cumulative immobilization at the end of 48 hour was recorded to be 0 % in control and daphnids exposed to 0.30, and 0.60 mg/L test concentrations whereas, 7.5 %, 17.5 %, 35 %, and 97.5 % was observed in daphnids exposed to 1.20, 2.40, 4.80 and 9.60 mg/L concentrations and 100 % was observed in daphnids exposed to 19.20 mg/L concentration. 

The EC50 of FAT 20290/C TE in Daphnia magna observed for a period of 48 hour was calculated as 4.40 mg/L with upper confidence limits of 6.64 mg/L and lower confidence limits of 2.93 mg/L and regression equation Y = 2.7293+3.5279 log (x).

The no observed effect concentration (NOEC) and the low observed effect concentration (LOEC) for 48 hours was calculated as 0.60 mg/L and 1.20 mg/L, respectively.