Registration Dossier
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EC number: 810-533-8 | CAS number: 330459-31-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48 h EC50 for Daphnia magna exposed to the test substance under static test conditions was >1.2 mg a.i./L and the NOAEC was 0.42 mg a.i./L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.2 mg/L
Additional information
A study was conducted to assess the toxicity of the test substance to Daphnia magna according to OECD Guideline 202 and US EPA OPPTS 850.1010, in compliance with GLP. Neonatal daphnids were exposed to a geometric series of five nominal test concentrations (0.17, 0.28, 0.47, 0.78 and 1.3 mg a.i./L), a negative control (dilution water) and a solvent control (DMF at 0.1 mL/L) for 48 h under static conditions without aeration. Two replicate test chambers were maintained in each treatment and control group, with 10 daphnids in each test chamber. Observations for immobility and other signs of toxicity were made at approximately 3.5, 24 and 48 h after test initiation. Samples for analytical determinations were collected from the batches of test solutions prepared for the treatment and control groups at the beginning of the test, and from each replicate per treatment and control group at 48 h (± 1 h). The mean measured test concentrations were 0.15, 0.25, 0.42, 0.75 and 1.2 mg a.i./L, representing 88, 91, 89, 96 and 92% of the nominal concentrations respectively. The results of the study were determined based on the mean measured concentrations. Daphnids in the control groups appeared normal throughout the test. Percent immobility in all treatment groups and controls were 0%. After 48 h of exposure, all surviving daphnids in the control groups appeared normal. There were observations of daphnia trapped in the water surface during the first 24 h of the test in both the negative and solvent controls as well as the 0.15, 0.25, 0.42 and 0.75 mg a.i./L treatment groups, but all appeared normal after being submerged beneath the water surface. This was considered incidental to the treatment. There were observations of lethargy at the end of the test in the 0.75 and 1.2 mg a.i./L treatment groups only. Under the test conditions, the 48 h EC50 forDaphnia magnaexposed to the test substance was >1.2 mg a.i./L and the NOAEC was 0.42 mg a.i./L (Thomas ST, 2013).
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