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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non-GLP Study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
Study performed before 2009.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-chlorobutan-2-one
EC Number:
223-834-9
EC Name:
3-chlorobutan-2-one
Cas Number:
4091-39-8
Molecular formula:
C4H7ClO
IUPAC Name:
3-chlorobutan-2-one
Constituent 2
Reference substance name:
3-Chloro-2-butanone
IUPAC Name:
3-Chloro-2-butanone
Test material form:
other: 3% (v/v) aqueous solution
Details on test material:
no info about substance purity in study report

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
40 male Wistar rats bred at the institute were used for testing.
Bodyweight 225 +/- 28 g.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
other:
Vehicle:
air
Details on inhalation exposure:
Vapour generation: in a 1.5L glass container at 50°C
Volume inhalation chamber: 20 L
Air circulation: 516 +/- 16 L/h
Temperature: 22-24°C
Humidity: approx. 60% (air was dried with CaCl2)
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
ca. 8 h
Concentrations:
0.000; 0.021; 0.043; 0.144; 0.190; 0.408; 0.720; 1.010 mL/m3 air
No. of animals per sex per dose:
5 male rats per dose (7 dose groups and 1 controll group)
Control animals:
yes
Details on study design:
14 days observation period after 8 hours inhalative (vapour) administration

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 0.5 other: mL/m3 air
Based on:
test mat.
Mortality:
1.010 mg/m3 air: 2/5 during inhalation; 2/5 on day 2; 1/5 on day 3; overall mortality 5/5
0.720 mg/m3 air: 5/5 on day 2
0.408 mg/m3 air: 1/5 on day 10
Clinical signs:
other: Control group and two lowest dose groups: no clinical signs observed. All other dose groups: weight loss; increasing coarse and untended fur. Highest dose group: laboured breathing, uncoordinated movement, sedation.
Body weight:
Weight gain approx. 2 g per animal in the three lowest dose groups.
Weight loss 4 / 7 / 17 g in the 0.144; 0.190; 0.408 mL/m3 dose groups.
Gross pathology:
Highest dose group: lung oedema found in the animals that died during the inhalative administration.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Remarks:
Migrated information
Conclusions:
Classification as Toxicity Category IV.
Executive summary:

The LC50 for male Wistar rats after vapour inhalation over 8 hours was found to be approx. 0.5 mL/m3 air (14 days observation period).