Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 292-587-7 | CAS number: 90640-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 Apr - 28 Apr 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study is complete, performed under GLP and valid. However no analysis was performed and thus the usability for risk assessment purposes is limited when assessing the bioavailable fraction.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- For the definitive test a stock solution of 0.10 g/litre was prepared by dissolving the test substance in deionized water.
This stock solution was titrated to a neutral pH. The selected test concentrations were prepared by dilution of this stock solution in the inoculated test medium. Based on the results of prelimanary experiments the nominal concentrations the definitive test were selected. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out with the unicellular green algae; Selenastrum capricornutum ATCC 22662. This strain was maintained on mineral salts agar slants in the light at room temperature.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22-24 °C
- pH:
- 6.9-7.8
- Nominal and measured concentrations:
- nominal: 0.5, 1.0, 2.0, 4.0, 8.0 mg/L
- Details on test conditions:
- The culturing apparatus is a cabinet in which the temperature is maintained between 22 and 24 °C, and continuous uniform illumination is provided in the spectral range of 400 to 700 run. The light intensity is in the range of 6000 to 10000 lux which is obtained by using seven 30 W fluorescent lamps (colour temperature of approximately 4300 K), at a distance of 0.35 m from the algal culture. The culture flasks were rotated continuously at 100 rpm to prevent sedimentation of the algae.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: both growth rate and biomass
- Details on results:
- The ErC50 and EbC50 (0-72 h) of tetraethylenepentamine are 6.8 and 2.1 mg/litre respectively. The NOEC determined of tetraethylenepentamime is 0.50 mg/litre.
- Reported statistics and error estimates:
- The EC50 values were computed from the best fitted line (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. The EC50 value calculated with the area under the growth curve is termed EbC50 (0-72 h) whereas the EC50 value calculated with the specific growth rate is termed ErC50. Confidence limits were computed based on Fieller's theorem. All computations were performed with the software package SAS.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 and EbC50 (0-72 h) of tetraethylenepentamine are 6.8 and 2.1 mg/litre respectively. The NOEC determined of tetraethylenepentamime is 0.50 mg/litre. The study was performed under GLP and meets all validity criteria. Only nominal test concentrations are presented which limits the use of this data when performing a risk assessment when looking at the bio-available fraction.
- Executive summary:
The toxicity of tetraethylenepentamine to exponentially growing S. capricornutum has been assessed over an exposure period of 72 hours. The algal inhibition test was performed according to the EEC/OECD (201) test guidelines. One minor deviation from the protocol of the algal inhibition test was introduced.
- The KH2PO4 and NaCO3 concentrations in the test medium were increased to 160 and 100 mg/litre, respectively, to maintain a constant pH.
- Prelimanary experiments to determine the concentration range are performed but not reported according to the guidelines.
In the definitive test the algae were exposed to the following nominal concentrations of 0.500, 1.000, 2.000, 4.000 and 8.000 mg/litre. The EbC50 (0 -72 h) and ErC50 of tetraethylenepentamine for S. capricornutum are 2.1 and 0.8 mg/litre, respectively. The NOEC determined of tetraethylenepentamine is 0.50 mg/litre. All test concentrations are based on nominal. All validity criteria are met and thus the study is valid.
However the usability of the presented data islimited due to the absence of actual measured concentrations and therefore no information on the bioavailable fraction.
Reference
Description of key information
EC50 (72 h): 6.8 mg/L (nominal)
NOEC (72 h): 0.5 mg/L (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 6.8 mg/L
- EC10 or NOEC for freshwater algae:
- 0.5 mg/L
Additional information
The acute toxicity of the substance to aquatic algae was tested in a study set up according to OECD guideline 201 and GLP. The freshwater algae Pseudokirchneriella subcapitata was exposed to nominal substance concentrations of 0.5, 1.0 2.0 and 8.0 mg/L.Analytical dose verification was not performed since the substance was expected to be stable under the tested conditions.
Based on the algal grwoth rate an EC50 (72 h) of 6.8 mg/L (nominal) was determined. The NOEC (72 h) was 0.5 mg/L for algal growth and biomass respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.