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EC number: 251-459-0 | CAS number: 33329-35-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of five male and five female rats were exposed for four hours in a 160-liter glass and stainless steel chamber to vapors and aerosols of ABBOTT-48832. The highest feasible nominal concentration was 22,5mg/L (actual concentration was not determined at this level). Both nominal and actual exposure concentrations were determined for the LC50 study. The exposure concentrations were as follows :
Actual Concentration Nominal Concentration
(mg/L) (mg/L)
1.1 3.6
1.7 6.3
2.3 8.3
2.4 9.6
2.8 11.3
Aerosols of the test material were generated by metering the liquid with a FMI pump into a spraying system atomizer. An air pressure of 10 psig with an airflow rate of 8 or 10 L/min was applied to the atomizer which aerosolized the liquid for rapid vaporization.
The vapors and aerosols emerging from the atomizer were diluted by the incoming chamber air to the desired concentration.
The size range of aerosol particles was 3.1 + 1.9 mcm.
Observations for signs of toxicity and mortality were made during the 4-hour exposure period and twice daily thereafter for 14 days.
Body weights were recorded on days 0 (prior to exposure), 1, 3, 7 and 14.
Animals that died during the study were necropsied. The surviving animals were necropsied at the end of the 14-day observation period. - GLP compliance:
- no
- Test type:
- fixed concentration procedure
Test material
- Reference substance name:
- N,N-bis[3-(dimethylamino)propyl]-N',N'-dimethylpropane-1,3-diamine
- EC Number:
- 251-459-0
- EC Name:
- N,N-bis[3-(dimethylamino)propyl]-N',N'-dimethylpropane-1,3-diamine
- Cas Number:
- 33329-35-0
- Molecular formula:
- C15H36N4
- IUPAC Name:
- (3-{bis[3-(dimethylamino)propyl]amino}propyl)dimethylamine
- Test material form:
- gas under pressure: refrigerated liquefied gas
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- other: Inhalation - vapour and aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- Aerosols of the test material were generated by metering the liquid with a FMI pump into a spraying system atomizer. An air pressure of 10 psig with an airflow rate of 8 or 10 L/min was applied to the atomizer which aerosolized the liquid for rapid vaporization.
The vapors and aerosols emerging from the atomizer were diluted by the incoming chamber air to the desired concentration. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- ca. 4 h
- Concentrations:
- Actual Nominal
(mg/L) (mg/L)
1.1 3.6
1.7 6.3
2.3 8.3
2.4 9.6
2.8 11.3 - No. of animals per sex per dose:
- 5
- Control animals:
- no
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 2 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 1.8 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- Up to 0-100% mortality in dosed groups.
- Clinical signs:
- other: Clinical signs observed were dyspnea, nasal discharge, salivation, evidence of red matter around the face, alopecia and dermal irritation.
- Body weight:
- All animals exposed demonstrated body weight loss sometime during the first week of the postexposure period.
- Gross pathology:
- At necropsy red lungs were observed in a few animals from each group. Scattered red patches on the lungs were also observed in a few animals from each group with the exception of Group 111, where the lesion was not observed. Black patches in the thymus was observed in three males and three females during the necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the nominal concentrations the calculated 4-hour LC50 for males was 7.4 mg/L, while for the females it was 6.4 mg/L. In terms of actual concentrations, the 4-hour LC50 for males was 2.0 mg/L, while for the females it was 1.8 mg/L.
- Executive summary:
Both nominal and actual exposure concentrations were determined for the LC50 study. Nominal concentrations were determined by weighing the amount of test material placed in the generator reservoir prior to exposure and then again following exposure. The difference in weight was divided by the total air passed through the chamber during the exposure period. Actual exposure concentrations were determined by standard gravimetric techniques.
The results indicated that nominal concentrations were higher than actual concentrations, e.g. 11.3 mg/L versus 2.8 mg/L for the highest concentration. Since B.P.>200°C, and vapour pressure=0.172 lb/inch3) this is a liquid material with low volatility, the actual measured concentrations seem more appropriate for evaluation of toxicity.
In conclusion, at actual exposure concentrations of 1.1-2.8 mg/L (nominal concentrations of 3.6-11.3 mg/L) for four hours produced signs of toxicity and deaths in rats. The combined male and female 4-hour LC50 values for actual measured concentrations and nominal concentrations were estimated to be 1.9 mg/L and 6.9 mg/L, respectively.
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